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- SYnAbs
- IR31-P-100
- 2025年07月12日
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文献和实验The history of isotype selection for therapeutic antibodies clearly demonstrates that solid understanding of the biologic effects mediated by immunoglobulin (Ig) subclasses IgG1, IgG2, IgG3, and IgG4 and their variants is a prerequisite
Isotype-specific immunostaining with mouse primary antibodies on mouse tissue
on mouse tissue are of IgG1 isotype. Detailed explanation of this method can be found in: Cattoretti G, Qing Fei. Application of the Antigen Retrieval Technique in experimental Pathology: from human to mouse
Screening lambda cDNA or genomic libraries
"NZY" plates for plating lambda libraries.In practice,LB medium works fine.Bacterial host strain- For most lambda strains use RY1073 or C600 HflA or LE392 for lambda hosts.Actually almost any e coli (like TG1)are ok for some lamda vectors,like lambda
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