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- 详细信息
- 文献和实验
- 技术资料
- 库存:
152
- 英文名:
Recombinant Rhesus Macaque Granulocyte Colony Stimulating Factor
- 保质期:
长期
- 供应商:
北京百奥莱博科技有限公司
- 保存条件:
冷冻(-20℃)
- 规格:
2μg
特别提示:包括重组猴G-CSF在内,本公司的所有产品仅可用于科研实验,严禁用于临床医疗及其他非科研用途!
产品名称:重组猴G-CSF
英文名称:Recombinant Rhesus Macaque Granulocyte Colony Stimulating Factor
产品货号:GS4456
产品规格:2μg
分子量:Approximately 18.9kDa,a single non-glycosylated polypeptide chain containing 174 amino acids.
储存条件:冷冻(-20℃)
概述
Granulocyte colony stimulating factor(G-CSF)is a pleiotropic cytokine and mainly produced by monocytes and macrophages upon activation by endotoxin,TNF-α and IFN-γ.Besides,many other cell types can secreted this protein after LPS,IL-1 or TNF-α activation,which are fibroblasts,endothelial cells,astrocytes and bone marrow stromal cells.Various carcinoma cell lines and myeloblastic leukemia cells can express G-CSF constitutively.G-CSF is cytokine that acts in hematopoiesis by controlling the production,differentiation,and function of 2 related white cell populations of the blood,the granulocytes and the monocytes-macrophages.In addition it may function in some adhesion or recognition events at the cell surface.
使用说明
This material is offered by Biorab Biotech for research,laboratory or further evaluation purposes.NOT FOR HUMAN USE.
技术规格
| 外观 | Sterile Filtered White lyophilized(freeze-dried)powder. |
| 纯度 | >98%by SDS-PAGE and HPLC analyses. |
| 来源 | Escherichia coli. |
| 生物活性 | Fully biologically active when compared to standard.The ED50 as determined by a cell proliferation assay using murine NFS-60 cells is less than 0.05ng/ml,corresponding to a specific activity of>2.0×107 IU/mg. |
| 成分 | Lyophilized from a 0.2μmfiltered concentrated solution in PBS,pH 7.4. |
| 内毒素 | Less than 0.1 EU/μg of rRhG-CSF as determined by LAL method. |
| 复溶 | We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom.Reconstitute in sterile distilled water or aqueous buffer containing 0.1%BSA to a concentration of 0.1-1.0mg/mL.Stock solutions should be apportioned into working aliquots and stored at ≤ -20 ℃.Further dilutions should be made in appropriate buffered solutions. |
| 氨基酸序列 | TPLGPASSLP QSFLLKCLEQ VRKIQGDGAA LQEKLCATYK LCHPEELVLL RHSLGIPWAP LSSCPSQALQ LTGCLSQLHS SLFLYQGLLQ ALEGISPELS PTLDTLQLDI ADFATTIWQQ MEDLGMAPAL QPTQGAMPAF TSAFQRRAGG VLVASHLQRF LELAYRVLRH LAQS |
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文献和实验个氨基酸残基和180个氨基酸残基组成,是由G-CSF的hnRNA分子经过可变剪接而来。体内174个氨基酸残基的G-CSF亚型含量更丰富,活性是180 个氨基酸残基的亚型的20倍。现通过重组DNA技术在大肠杆菌中表达的非糖基化的G-CSF,含有175个氨基酸残基,分子量18.8KD。 临床上,重组人G-CSF(rhG-CSF)用于治疗实体瘤和非髓性白血病化疗后引起的中性粒细胞减少症,依据的原理即为G-CSF可促进中性粒细胞的生成,从而减少化疗后由于中性粒细胞减少导致的各种感染。rhG-CSF在此类
,G-CSF)是血管内皮细胞、单核细胞和成纤维细胞合成的糖蛋白。能促进中性粒细胞成熟;刺激成熟的粒细胞从骨髓释出;增强中性粒细胞趋化及吞噬功能。对巨噬细胞、巨核细胞影响很小。现用的G-CSF为基因重组产品。皮下注射5~10μg/kg,4~6小时达血浓高峰,高于10μg/kg,血浓可维持16小时。静静滴注(60μg/kg,20~30分滴完),t 1/2 为0.75~7.2小时。1987年起用于肿瘤化疗、放疗引起骨髓抑制,也用于自体骨髓移值。对再生障碍性 贫血 、骨髓
佚名 维生素B 4 、鲨肝醇等作为升白细胞药应用多年,但疗效较差。基因重组及克隆技术则为集落刺激因子的生产和应用创造了条件。 粒细胞集落刺激因子 粒细胞集落刺激因子(granulocyte colony-sitmulating factor,G-CSF)是血管内皮细胞、单核细胞和成
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