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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
低温
- 保质期:
保质期内
- 英文名:
MKM
- 库存:
充足
- 供应商:
北京美科美生物技术开发有限公司
- CAS号:
来电咨询
- 规格:
10 ml
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文献和实验The baculovirus expression system represents an elegant means whereby high-level expression of foreign genes can be achieved using eukaryotic insect cells (1,2) . Viruses from the Baculoviridae subfamily of insect viruses, usually Autographa
lution of GST-Fusion Protein from Glutathione Agarose
lution of GST-Fusion Protein from Glutathione Agarose 1. Wash the GST-fusion protein glutathione-agarose complex once with 1% Triton X-100 in PBS. 2. Resuspend the complex in 1% Triton X-100 in PBS. 3. Prepare a small column
Extraction of DNA from Agarose Gels
, to destabilize the agarose gel. The DNA is subsequently bound to a substrate (e.g., an anionic resin) and washed to remove impurities prior to elution of the DNA from the substrate. Other methods include hot phenol extraction of the DNA from the gel. The use
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