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文献和实验Extraction of DNA from Agarose Gels
, to destabilize the agarose gel. The DNA is subsequently bound to a substrate (e.g., an anionic resin) and washed to remove impurities prior to elution of the DNA from the substrate. Other methods include hot phenol extraction of the DNA from the gel. The use
Agarose-Formaldehyde Electrophoresis
Agarose-Formaldehyde Electrophoresis RNA electrophoresis under denaturing conditions in 2.2M formaldehyde is performed according to Maniatis et al., (1982) using the MOPS buffer system. RNA under these conditions is fully denatured
Northern Hybridization of RNA Fractionated by Agarose-formaldehyde Gel
The protocol is divided into three sections: Electrophoresis of an RNA preparation under denaturing conditions in an agarose-formaldehyde gel Transfer of the RNA from the gel to a nylon or nitrocellulose membrane by upward capillary transfer
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