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文献和实验E.Z.N.A. Cycle-Pure Kit Spin Protocol
of the sample at 260nm and then at 280nm. The DNA concentration is calculated as follows: 260 DNA concentration = A x 50 x (Dilution Factor) ug/ml Fragments greater than 500bp in length can routinely be purified at > 80% yield. 260 Bands ranging
培养细胞RNA提取--RNA Isolation Protocol-Cultured Cell Samples
not to disturb the pellet. Allow the pellet to air dry for 10-15 minutes. Do not allow the pellet to completely dry out. Resuspend the pellet in 200-400ul of Ultra-pure water. Spec the RNA at OD 260 to determine the ug amount of RNA. Add appropriate
Viral RNA extraction-Molecular Biology
to extract viral RNA out from a formalin fixed, paraffin embended viral infected tissue. However after extraction, the ectracted RNA is either not pure (ratio 1.27-1.35) or RNA concentration too little (6 ug/ml).I run with a RT-PCR togather with positive
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