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文献和实验300, ETS1, ETS2, FKBP1B, FLJ14639 (NIP45), FOS, FOSL1, FOXO1A, FOXO3A, GATA3, GATA4, GRLF1, ICOS, IRF1, ISGF3G, JUN, JUNB, MADH4, MAPKAPK2, MAPKAPK3, MAX, MEF2A, MEF2B, MEF2C, MEF2D, MHC2TA, MKNK1, MLLT7, MYC, MYF5, NFKB2, NFKBIB, NFKBIE, NR4A2, PCNA, RAF
Small scale His-Tag fusion protein purification under denaturative conditions
plastic tube. Wash with 2 x 1.5ml H2 O and 2x 1.5ml equilibration buffer (washing: mix, spin 3min 3500rpm, discharge supernatant). Buffers Lysis buffer: 50mM Na2 HPO4 pH 8.0, 0.3M NaCl, 1mM PMSF (or protease inhibitor cocktail
自然染色质免疫沉淀实验设计（Native Chromatin Immunoprecipitation (N-ChIP)）
1. The preparation of native chromatin from cultured human cells 1.1.Cultured cells (e.g. HL-60 or lymphoblastoids) are grown to a density of approximately 1 x 106 cells/ml until they are inlog phase. 1.2.Harvest cells: centrifuge samples
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