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文献和实验it should an 18-22-mer with an annealing temperature of around 56℃, where a G/C = 4℃ and an A/T = 2℃.Protocol1. Place approx. 1ug of total RNA (~10ng polyA RNA) in an RNase-free eppendorf, make up to 10μl with pure water. Add 1μl of T17AP (0.5ug/ul).2. Heat
approx. 1ug of total RNA (~10ng polyA RNA) in an RNase-free eppendorf, make up to 10ul with pure water. Add 1ul of T17AP (0.5ug/ul). 2. Heat this to 70°C for 10 minutes, then place on ice for 5 minutes. 3. Spin pulse the tube and then add
实验步骤 1. Place approx. 1ug of total RNA (~10ng polyA RNA) in an RNase-free eppendorf, make up to 10ul with pure water. Add 1ul of T17AP (0.5ug/ul). 2. Heat this to 70°C for 10 minutes, then place on ice for 5 minutes
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