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文献和实验) should be performed for optimization. Add 100l of the cell suspension to each well. For each condition, use triplicate wells. Add soluble antibody in 100l to each well. Titrate antibodies for optimal performance in the assay conditions used. If isolated T
Collection, Separation, and Activation of Human T Lymphocytes
(3 ). In particular, activation of antigen-specific CD4+ T cells of the Th2 subset in the lungs, resulting in interleukin (IL)-5 secretion, plays a major role in allergic inflammation of the airways (4 ). T-cell activation requires two signals, one provided
T-Cell Activation Using mAb to CD3
splenic T-cells and human peripheral T cells stimulated via CD3. Critical parameters include cell density, antibody titer and activation kinetics. Materials 1X sterile PBS Anti-mouse CD3e, Clone 145-2C11 (Functional Grade, Cat. No. 16
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