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文献和实验CCMB buffer to make chemical competent cell
transformation efficiency. 6. 5x Ligation Adjustment Buffer Intended to be mixed with ligation reactions to adjust buffer composition to be near the CCMB80 buffer KOAc 40 mM (40 ml/liter of 1 M KOAc solution, pH 7.0) CaCl2 400 mM (200 ml/l of a 2 M
SQ Blood DNA Maxi Protocol for 4-10 ml whole blood
15 ml microcentrifuge tubes 3. Water baths preset at 37°C 4. Paper towels 实验步骤 1. Add one volume of whole blood (or bone marrow) to a nuclease-free 50 ml centrifuge tube containing 3 volume of Buffer ERL. Mix
Gel Mobility Shift Assay Conditions -Mg/EDTA in Gel and Buffer
58 to 0.1%.Store dilution buffer at -70 degrees.Proteins are diluted in dilution buffer and quick frozen on dry ice.Thaw proteins on ice.Proteins are typically stable to multiple repeated freeze thaw.5x binding buffer 1 ml20% glycerol 400 microliters
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