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文献和实验A c-Kit (2B8) FITC-IgG2b, 01904D FITC-IgG2b Isotype control, 11184C Fc RII block (clone 2.4G2), 01241D Media: Heat inactivated FBS (56°C x 30 min) PBS + 2% heat-inactivated
Mouse anti-Human CD37 mAb,PE 50T/100Tsets 1000/1500 L0093 Mouse anti-Human CD38 mAb,FITC 50T/100T 800/1200 L0094 Mouse anti-Human CD38 mAb,PE
Single-molecule analysis of DNA replication by molecular combing
The protocol described below is meant to detect BrdU incorporation in yeast cells arrested in early S phase with hydroxyurea (HU). It can be easily adapted to monitor ongoing DNA replication in asynchronous yeast cultures or to analyze DNA replication
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