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- 2025年07月06日
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文献和实验CCMB buffer to make chemical competent cell
transformation efficiency. 6. 5x Ligation Adjustment Buffer Intended to be mixed with ligation reactions to adjust buffer composition to be near the CCMB80 buffer KOAc 40 mM (40 ml/liter of 1 M KOAc solution, pH 7.0) CaCl2 400 mM (200 ml/l of a 2 M
LABELING GC-GLOBULIN WITH FLUORESCEIN
Materials 1.2 mM PIPES,pH 7.0,500 ml. 2.Gc-globulin (Calbiochem 345802),1 mg. 3.CFSE (Carboxyfluorescein succinimidyl ester; Molecular Probes). 4.Lysine,100 mM in buffer 1,10 ml. 5.G25-150 desalting column,1 cm diameter.Equilibrated with buffer
An Economic PCR Enhancer for GC-Rich PCR Templates
several different polymerases The 5x concentrated Combinatorial Enhancer Solution (CES) contains: 2.7 M betaine 6.7 mM DTT 6.7% DMSO and 55 μg/ml BSA Add this solution to your PCR and see if it works Some hints if your PCR
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