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文献和实验A c-Kit (2B8) FITC-IgG2b, 01904D FITC-IgG2b Isotype control, 11184C Fc RII block (clone 2.4G2), 01241D Media: Heat inactivated FBS (56°C x 30 min) PBS + 2% heat-inactivated
Chick Chorioallantoic Membrane (CAM) Assay
into 100 mm2 tissue culture coated Petri dishes under aseptic conditions. The embryos were then returned to a humidified 38°C incubator2 for 7-9 additional days. Mesh assay Substrates Vitrogen (Collagen Biomaterials, Palo Alto, CA) was diluted
secondary antibody review -- data from 99 publications
IgG Alexa Fluor 488 1:250 Molecular Probes 10 goat Cy3 immunocytochemistry detect NMDA receptors in HEK293 cells Jackson ImmunoResearch Laboratories 10 FITC immunocytochemistry 1:100 detect phospho-CREB in human colonic
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