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文献和实验8.0. Resuspend the final washed nuclear pellet in ice cold distilled water to a final volume of 100 ml and allow to swell overnight at 4° C. Gently stir the mixture on the following day. This solution is the pure chromatin to be used
Non-fluorescent protein/RNA double-labeling
the embryos with 3% hydrogen peroxide in methanol for 15 min. Rinse well in methanol. 3. Wash 3 X 30 minutes with PBTH (filtered 1 X PBS, 0.1% Tween-20, 50 ug/ml heparin, and 250 ug/ml tRNA). You can also include 1% BSA providing you know it is ultra pure
Non-fluorescent protein/RNA double-labeling
, 50 ug/ml heparin, and 250 ug/ml tRNA). You can also include 1% BSA providing you know it is ultra pure/RNase-free. With our reagents, we find we get similar results without it. 4. Incubate with the primary antibody (diluted appropriately in PBTH
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