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文献和实验Nat Med：个体化肿瘤疫苗突破，特异性 T 细胞持续 4.5 年，患者存活率 100%
论文链接：[1] Zhuting Hu et al. Personal neoantigen vaccines induce persistent memory T cell responses and epitope spreading in patients with melanoma. Nature Medicine, 2021, doi:10.1038/s41591-020-01206-4.[2] Patrick A. Ott et al. An immunogenic personal
Biotin labeling and hybridization of probe to chromosomes
Biotin labeling and hybridization of probe to chromosomes Suggested chemicals: Nick Translation Kit, Enzo Diagnostics Cat. No. 42803. Biotin labeling of plasmid or genomic DNA. Remove slides
Preparation and purification of biotin-labeled probe for ISH
is collected in the 1.5 ml tube The final volume of probe should be close to 55 µl. Usually, 5 µl of such a biotin-labeled probe (1 µg/55 µl = 18 ng/µl) is enough for one slide, and the probe can be used to process 100 slides. The size of the probe
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