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文献和实验Zymolyase Dissolve in 10% glucose. Store aliquots (25 µl) at 20o C in common freezer for stock solutions Use once and dispose Comments: alternative is to dissolve in SCE, 40% gycerol, 20 mg/ml final. Can use
Preparation of Microinjection Buffer
EDTA, pH 8.0 (autoclaved) 100 mM NaCl 1 ml of 5 M NaCl (autoclaved) 1x Polyamines 50 microliters 1000x Polyamines mix Autoclaved H2O up to 50 ml NOTE: Prepare fresh and discard unused microinjection buffer.
Mag-Bind Blood DNA Maximum Yield Protocol (2ml-6ml)
实验试剂 1. Absolute ethanol (96%-100%) 实验设备 1. Nuclease-free 50 ml centrifuge tube 2. Water bath, incubator or heating block preset at 65°C 3. Magnetic separation device for 50 ml tube
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