Phosflow Perm/Wash Buffer I 12

SQ Blood DNA Maxi Protocol for 4-10 ml whole blood

15 ml microcentrifuge tubes 3. Water baths preset at 37°C 4. Paper towels 实验步骤   1. Add one volume of whole blood (or bone marrow) to a nuclease-free 50 ml centrifuge tube containing 3 volume of Buffer ERL. Mix

whole mount preparations

of X-gal staining buffer with 1 mg/ml X-gal (make 40mg/ml stock in DMF, store at -20OC; don't use if discolored). Incubate at 30OC for 24 -48 hrs, or less if expression is strong 5. Clear in acetone, rehydrate and stain in carmine alum O/N.

RNA-biotin based pulldown assays for the detection of siRNA targeted genomic regions and siRNA directed histone modifications (PROT32)

   3.75ml 4M NaCl       3ml 10% Triton X (Tx-100)       300µl 10% NAD       21.45ml H20 ChIP wash buffer       30ml final volume:       150µl 2M Tris       1.7ml 4.4M LiCl       60µl 0.5M EDTA       1.5ml 10% NAD       1.5ml 10% NP-40       25.09ml