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文献和实验Fluorochrome Absorption and Emission Spectra
. Using a 650 nm longpass filter will give optimum detection for this fluorochrome. The emitted light is collected in the fluorescence-3 (FL3) channel. PerCP-Cy5.5 is recommended for use with stream-in-air flow cytometers. APC-Cy7
Fluorochrome Absorption and Emission
. Using a 650 nm longpass filter will give optimum detection for this fluorochrome. The emitted light is collected in the fluorescence-3 (FL3) channel. PerCP-Cy5.5 is recommended for use with stream-in-air flow cytometers. APC-Cy7
Cell Surface Immunofluorescence Staining Protocol
-CD4-Biotin, and anti-CD8-APC) and incubate on ice for 15-20 minutes in the dark. 9) Wash 2X with at least 2 ml of Cell Staining Buffer by centrifugation at 350 x g for 5 minutes. 1) If using a purified primary antibody, resuspend
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