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文献和实验Methods for DNA Barcoding Photosynthetic Protists Emphasizing the Macroalgae and Diatoms
1 gene) as the primary barcode marker for brown and red algae; rbcL -3P (the 3′ region of the plastid large subunit of ribulose-l-5-bisphosphate carboxylase/oxygenase) as the primary barcode marker for diatoms; and tufA (plastid elongation factor Tu gene
Melting Curve giving flutating Ct values-Real-Time PCR
everything else to make sure you're not getting degradation / inhibition somewhere? The efficiencies aren't great I admit, I know we have to make sure the efficiency of the housekeeping gene and the experimental gene should be similar but I can't
Degenerate PCR is in most respects identical to ordinary PCR, but with one major difference. Instead of using specific PCR primers with a given sequence, you use mixed PCR primers. That is, if you do not know exactly the sequence of the gene
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