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- 详细信息
- 文献和实验
- 技术资料
- 亚型:
Rat IgG2b, κ
- 形态:
液体
- 保存条件:
This product can be stored at 2-8°C for 12 months. Please protected from prolonged exposure to light and do not freeze.
- 克隆性:
Monoclonal
- 标记物:
PE/Cyanine5
- 适应物种:
Mouse
- 保质期:
12个月
- 目录编号:
E-AB-F1136UG
- 级别:
科研
- 库存:
999
- 供应商:
武汉伊莱瑞特生物科技股份有限公司
- 宿主:
Rat
- 应用范围:
FCM
- 抗体英文名:
PE/Cyanine5 Anti-Mouse CD45 Antibody[30-F11]_货号:E-AB-F1136UG
- 抗体名:
PE/Cyanine5 Anti-Mouse CD45 Antibody流式抗体[30-F11]_货号:E-AB-F1136UG
- 规格:
100μg/25μg
| 规格: | 100μg | 产品价格: | ¥2280.0 |
|---|---|---|---|
| 规格: | 25μg | 产品价格: | ¥840.0 |
货号:E-AB-F1136UG
| Background |
CD45 is a 180-240 kD glycoprotein also known as the leukocyte common antigen (LCA), T200, or Ly-5. It is a member of the protein tyrosine phosphatase (PTP) family, expressed on all hematopoietic cells except mature erythrocytes and platelets. There are different isoforms of CD45 that arise from alternative splicing of exons 4, 5, and 6, which encode A, B, and C determinants, respectively. CD45 plays a key role in TCR and BCR signal transduction. These isoforms are very specific to the activation and maturation state of the cell as well as cell type. The primary ligands for CD45 are galectin-1, CD2, CD3, CD4, TCR, CD22, and Thy-1.
|
| Alternate Names |
Receptor-type tyrosine-protein phosphatase C;Ptprc;L-CA;Ly-5;T200;CD45;
|
| Swissprot |
P06800
|
| Gene ID |
19264
|
| Clone No |
30-F11
|
| Host |
Rat
|
| Reactivity |
Mouse
|
| Application |
FCM
|
| Isotype |
Rat IgG2b, κ
|
| Isotype Control |
PE/Cyanine5 Rat IgG2b, κ Isotype Control[LTF-2]
|
| Conjugation |
PE/Cyanine5
|
| Conjugation Information |
PE/Cyanine5 is designed to be excited by the Blue (488 nm), Green (532 nm) and yellow-green (561 nm) lasers and detected using an optical filter centered near 670 nm (e.g., a 690/50 nm bandpass filter).
|
| Storage Buffer |
Phosphate buffered solution, pH 7.2, containing 0.09% sodium azide and 1% BSA.
|
| Storage |
This product can be stored at 2-8°C for 12 months. Please protected from prolonged exposure to light and do not freeze.
|
| Expiration date |
12个月
|
| Shipping |
冰袋
|
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文献和实验自2012年第一篇引用文献问世,至2023年8月,Elabscience已协助各地科研工作者发表超13,000篇SCI文献,包括行业顶/级期刊Nature Medicine, Nature, Cell, Signal Transduction and Targeted Therapy, Circulation, Molecular Cancer, Immunity, Advanced Materials等,总影响因子(IF)59472.59,其中IF≥30的文献超30篇,最高影响因子为66.849,发文单位包含北京大学、中国科学技术大学、浙江大学、斯坦福大学、耶鲁大学、柏林夏里特大学等国内外各大高校。
每年引用文献数量持续增长,保持逐年30%+高增长率,可以预见,Elabscience产品将帮助更多科研工作者发表高质量SCI论文。
文献详情查看>>>>


69、CD38 等活化指标,及 IFN-γ、IL-4、IL17A 等细胞因子,则需要设置同型对照,实现阴阳性细胞群的良好区分。 CD4 CD25 (2)在某些特殊情况下,如单核细胞被 PE/Cyanine 7、APC/Cyanine 7 等串联染料标记的抗体染色时,使用同型对照抗体是十分有必要的。单核细胞一般倾向于与 Cyanine7 等染料非特异性结合,使用同型对照抗体有助于获得理想结果。 (3)不清楚抗原表达情况的时候,一定要使用同型对照来界定阴性细胞群。 (4)胞内染色时
Models and Analysis of Atherosclerosis, Restenosis, and Aneurysm Formation in the Mouse
Leukocytes CD45 Rat 30F11
、CD14-PE、CD19-PE、CD3-PE、CD66b-PE、CD56-PE-Cy7、CD11c-APC、 CD45-AF700、CD38-APC-AF750(每次测试 5μL),以及 CD16-FITC(每次测试 20 μL),并在冰上孵育 30 分钟。10. 在 4℃ 条件下,300 x G 离心细胞 5 分钟。11. 吸取上清液。12. 重悬到 500 μL 染色缓冲液中。13. 通过 CytoFLEX 获取结果。通过 CytoFLEX 进行数据采集:1. 创建新补偿值。2. 如适用
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