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- 文献和实验
- 技术资料
- 保存条件：
-20°C, protect from light
- 英文名：
7-AAD
- 库存：
货期：1-2天
- 供应商：
MedChemExpress LLC
- CAS号：
7240-37-1
- 规格：
1 mg/5 mg/10 mg
| 规格： | 1 mg | 产品价格： | ￥2300.0 |
|---|---|---|---|
| 规格： | 5 mg | 产品价格： | ￥5200.0 |
| 规格： | 10 mg | 产品价格： | ￥8450.0 |
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7-Aminoactinomycin D
CAS No. : 7240-37-1
MCE 国际站：7-Aminoactinomycin D
产品活性：7-Aminoactinomycin D (7-AAD) 是一种荧光 DNA 染料，是一种有效的 RNA 聚合酶 (RNA polymerase) 抑制剂。7-Aminoactinomycin D 选择性结合到 DNA 的 GC 区域，还具有抗菌作用。
研究领域：Anti-infection | Cell Cycle/DNA Damage
作用靶点：DNA/RNA Synthesis | Bacterial | DNA Stain | Antibiotic
In Vitro: 7-Aminoactinomycin D (7-AAD) is a DNA dye that distinguishes viable, apoptotic, and late apoptotic/dead cells in flow cytometry. 7-Aminoactinomycin D staining with 5 μg/mL, 10 μg/mL, and 20 μg/mL, but not with 1 μg/mL, is suitable for quantification of apoptosis in flow cytometry.
?7-Aminoactinomycin D is frequently used to stain and exclude dead cells in flow cytometry at low concentrations (0.5-5 μg/mL). At higher concentrations (10-20 μg/mL), 7-Aminoactinomycin D has also been used to distinguish between viable cells (7-AADnegative) and apoptotic cells (7-AADdim) or dead cells (7-AADbright) using the fact that permeability of the cell membrane, and hence fluorescence intensity, is low in early apoptotic cells and high in late apoptotic and dead cells.
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文献和实验指DNA双链的局部，由具有互补性单链 DNA与之结合所产生的环状结构。当 DNA复制开始，在原来的双链中仅一方被新合成的短 DNA单链被置换的情况下可以见到（为 Displacement loop之简称）。可通过人工使 DNA单链结合来制成此结构。由 RNA单链所产生的类似结构称为 R环。
of each component.2D gel electrophoresis is generally used as a component of proteomics and is the step used for the isolation of proteins for further characterisation by mass spectroscopy. In the lab this technique is used for 2 main purposes, firstly for the large
of each component. 2D gel electrophoresis is generally used as a component of proteomics and is the step used for the isolation of proteins for further characterisation by mass spectroscopy. In the lab this technique is used for 2 main purposes, firstly
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