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文献和实验, and 20 mMTris-HCl, pH 7.5, sterile filtered. 5. Syringe (30–50 mL) with 0.2-μm pore size filter. 2.3.2. Step 2: IMAC 1. Sample: Pooled Wnt3A containing fractions from Section2.3.1 . 2. HiTrap? Chelating, 1-mL column (GE Healthcare, Piscataway
Company � All rights reserved. GE Healthcare AB, a General Electric Company. GE Healthcare Europe GmbH Munzinger Strasse 5 D-79111 Freiburg Germany GE Healthcare UK Ltd. Amersham Place Little Chalfont Buckinghamshire, HP7 9NA UK GE
Metallomics Using Inductively Coupled Plasma Mass Spectrometry
) 1 ml phenyl Sepharose column (e.g., HiTrap Phenyl HP; GE Healthcare) 1 ml Mono Q 5/50 GL column (GE Healthcare)
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