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- 17088504
- 2025年07月10日
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文献和实验Evaluating Modulators of “Regulator of G‐protein Signaling” (RGS) Proteins
7.5), 1 mM EDTA, 10 mM MgCl 2 , 10 µM GTP, 10 µg/ml GDP antibody, and 2 nM fluorescent nucleotide tracer; for the protocol below, this will be prepared as a 1.1× solution (18 µl added to a final 20 µl
(Cibacron Blue F3G-A coupledto Sepharose), ~100 mL packed into an empty column with100–200 mL of bed volume. 3. Binding Buffer: 1% (w/v) CHAPS, 150 mM KCl, and 20 mMTris-HCl, pH 7.5, sterile filtered. 4. Elution Buffer: 1% (w/v) CHAPS, 1.5 M KCl
.g. 20% ethanol, at neutral pH. 9. Ordering Information Product Pack size Code No. Benzamidine Sepharose 6B 25 ml 17-0568-01 10. References 1. Purification and characterization of a trypsin-like serine proteinase from rat brain slices
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