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- 详细信息
- 文献和实验
- 技术资料
- 库存:
进口期货
- 供应商:
上海经科化学科技有限公司
- 抗体英文名:
Goat anti-Chicken IgY (H&L), ALP conjugated
- 规格:
1mg
| Immunogen |
| purified chicken IgY, whole molecule |
|
Host Goat
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Clonality Polyclonal
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Purity Affinity purified goat IgG
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Format Liquid
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Quantity 1 mg
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Storage Non-diluted antibody is stable for 4 years at 2-8°C. For storage at -20°C dilute antibody solution with an equal volume of glycerol to obtain final glycerol concentration of 50 % to prevent loss of enzymatic activity. Such solution will not freeze in -20°C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard. Be sure to mix well but without foaming.
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Tested applications ELISA (ELISA), Immunohistochemistry (IHC), Western blot (WB)
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Recommended dilution 1 : 10 000 -1 : 100 000 (ELISA), 1 : 500 -1 : 2000 (IHC), 1 : 10 000-100 000 (WB)
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名称:Goat anti-Chicken IgY (H&L), ALP conjugated
货号:AS09 606
品牌:Agrisera
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文献和实验IgG(H+L) 产品详情同时,Dylight 405作为Dylight系列的荧光团,具有很多优势,包括更高的荧光强度、更好的光学稳定性和特异性、更高的性价比等。具体见荧光二抗的选择——Dylight荧光与传统荧光标记的比较。EarthOx的部分Dylight 405荧光抗体产品如下:山羊抗大鼠IgG二抗, 蓝色DyLight 405荧光标记 Dylight 405 AffiniPure Goat Anti-Rat IgG(H+L) 产品详情兔抗山羊IgG二抗, 蓝色DyLight 405荧
T-cell dependent antigen specific in situ staining (Pe)
HRP-conjugated secondary Ab choice: 10- add the HRP-conjugated Goat anti rabbit (Vector 50 to 100 µl, dilution 1:100) and incubate for 30’. Swine anti-rabbit (Dako) can be used at 1:300 dilution. If you use this step for tyramide
5- add the AP conjugated tertiary antibody (50 to 100 µl) and incubate for 15 min. The tertiary antibody should be absorbed against human serum; if not add 1% human serum before use. SBA Goat anti mouse AP or Goat anti Rabbit AP can be used
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