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- 详细信息
- 文献和实验
- 技术资料
- 库存:
进口期货
- 供应商:
上海经科化学科技有限公司
- 抗体英文名:
GST class-phi | Glutathione S tranferase
- 规格:
100ul
| Immunogen |
| KLH-conjugated synthetic peptide derived from Arabidopsis thaliana UniProt: P46422, TAIR: At4g02520 |
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Host Rabbit
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Clonality Polyclonal
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Purity Serum
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Format Lyophilized
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Quantity 100 µl
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Reconstitution For reconstitution add 100 µl of sterile water.
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Storage Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
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Tested applications ELISA (ELISA), Western blot (WB)
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Recommended dilution 1 : 8000 (ELISA), 1 : 1000 (WB)
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Expected | apparent MW
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| 24 | 26 kDa |
名称:GST class-phi | Glutathione S tranferase
货号:AS09 479
品牌:Agrisera
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文献和实验,只要互作蛋白间具有相互结合的结构域基础即可实验证明;Co-IP 实验是细胞内高表达目的蛋白,并通过 IgG 球珠-抗体-蛋白复合物体系,分离出互作的蛋白复合物,是细胞内的体内反应结合。2. GST pull-down 实验优点① 可验证蛋白质-蛋白质之间的直接互作。② GSH 谷胱甘肽偶联球珠亲和力强,洗脱纯度高。3. GST pull-down 实验缺点① 验证互作是在试管中进行的生化反应,不能够完全反应细胞内蛋白真实互作状态。② 融合表达的 GST 标签,肽链较长,可能会改变原目的蛋白的原有的折叠结构。
mL PBS 1X 4- Centrifuge (1pulse at 500g) 5- Resuspend in 10 mL PBS 1X (stable for one month at 4 _ C) b) Extract preparation: 1- Transform BL21 or any appropriate bacterial strain with your GST-fused cDNA. Always use freshly transformed cells
with 1/2 volume of 50% GST beads at 4C rocking on the Nutator for 30-60 minutes. 50 ml conical Falcon tubes work well. Pour into a BioRad Column and drain lysate. Save 100 ul of depleted lysate. Load 2.5 ul for SDS-PAGE. Wash beads with 3 column volumes
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