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文献和实验RAT/MOUSE GROWTH HORMONE ELISA KIT
实验原理 This assay is a Sandwich ELISA based, sequentially, on: 1) capture of rat or mouse Growth Hormone molecules from samples to the wells of a microtiter plate coated by a pre-titered amount of anti-Growth Hormone
chain monoclonal antibody conjugated to horse-radish peroxidase (LO-MK1, LO/IMEX) 8. reveal with o-phenylenediamine dihydrochloride (OPD) in revelation solution. B. SOLUTIONS 1. PBS-PF (paraformaldehyde), 2% 2. PBS-tween 20, 0.05% 3. glycine (x
prepare platelets from control and immunized mice distribute 2.5 x106 platelets per well on F-bottomed MaxiSorp Nunc-Immuno microplates (GibcoBRL, Roskilde, Denmark), centrifuge at 3000 rpm, 7 min, 10ѓC. fix with PBS-PF
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