Invitrogen Ki-67 Monoclonal An

Invitrogen

Ki-67 Monoclonal Antibody (SP6)

货号 MA5-14520
500 µL
CNY 5,127.00
 

Ki-67 Antibody (MA5-14520)

PloS one 2014 - Figure 3 Minnelide leads to tumor regression in xenograft mouse models. In two xenograft mouse models, A549 (right panel) and NCI-H460 (left panel), tumor volumes were compared between minnelide treated (n = 10) and untreated groups (n = 10). Five days after tumor injection, mice began receiving daily intraperitoneal injections of minnelide at 0.42 mg/kg mouse weight. Control animals were injected with equi..

产品详细信息

MA5-14520 targets Ki-67 in Immunohistochemistry (P) applications and shows reactivity with Human samples. Staining of formalin-fixed tissues requires boiling tissue section in 10 mM citrate buffer, pH 6.0 for 10-20 minutes followed by cooling at room temperature for 20 minutes. Suggested positive controls include spleen, tonsil or breast carcinomas.

The MA5-14520 immunogen is a synthetic peptide derived from the human Ki-67 protein.

靶标信息

Ki-67 is a nuclear protein that is expressed during various stages in the cell cycle, particularly during late G1, S, G2, and M phases. The protein has a forkhead associated domain (FHA) through which it associates with euchromatin at the perichromosomal layer, the centromeric heterochromatin, and the nucleolus. Ki-67 is shown to have a cell cycle dependent topographical distribution with perinucleolar expression at G1, expression in the nuclear matrix at G2, and expression on the chromosomes during M phase. Ki-67 is commonly used as a proliferation marker because it is not detected in G0 cells, but increases steadily from G1 through mitosis. Ki-67 antibodies are useful in establishing the cell growing fraction in neoplasms. In neoplastic tissues, the prognostic value is comparable to the tritiated thymidine-labelling index. The correlation between low Ki-67 index and histologically low-grade tumors is strong. Ki-67 is routinely used as a neuronal marker of cell cycling and proliferation.

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