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- 详细信息
- 文献和实验
- 技术资料
- 库存:
60
- 供应商:
深圳子科生物科技有限公司
- 规格:
100 Reactions
咨询热线:13924667705 QQ 2811391951苏经理。
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| G013-dye | 2X PCR Taq MasterMix with dye | 5.0 ml (200 Rxns) | |
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| G277 | TaqFast DNA Polymerase | 250 U (50 µl) | |
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| G280 | 2X PCR TaqFast MasterMix | ||
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文献和实验of mRNA) or random hexamers; the kit used here contains both kinds); nucleotides for DNA synthesis (dNTPs); MgC2 and buffers required by the enzyme, which are all present in the 5x Reaction Mix. Materials * iScript cDNA Synthesis Kit (BIORAD
分子的RNA(200nt以上),小分子RNA往往被淘汰掉,因而不适用于小分子RNA的分离纯化。有机溶剂抽提能够较好的保留小分子RNA,但是后继的沉淀步骤比较费时费力。MirVana miRNA Isolation Kit是采用玻璃纤维滤膜离心柱(glass fiber filter,GFF),既能够有效富集10mer以上的RNA分子,又能够兼备离心柱快速离心纯化的优点,是一个不错的选择。对于特别稀有的分子,由于需要分离大量RNA而导致高背景而降低灵敏度,还可以进一步富集10mer到200bp的小分子RNA
methylation analysis, PCR, and multiplex PCR. The purified total RNA is ready to use in any application, such as RT-PCR, real-time RT-PCR, differential display, cDNA synthesis, Northern, dot, and slot blot analyses, and microarray analysis. The kits form
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