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| PCR试剂 | G008 | Taq DNA Polymerase | 5000u |
| G126 | Taq DNA Polymerase | 10000U | |
| G009 | Taq DNA Polymerase | 1000U(200 μl) | |
| G013 | 2X PCR Taq MasterMix | 5.0 ml (200 Rxns) | |
| G013-dye | 2X PCR Taq MasterMix with dye | 5.0 ml (200 Rxns) | |
| G472 | Safe-Green ; 2X PCR Taq MasterMix | 5.0 ml (200 Rxns) | |
| G012 | Taq Plus DNA Polymerase | 250 U (50 µl) | |
| G040 | Taq Plus DNA Polymerase | 1000U(200 µl) | |
| G014 | 2X PCR Taq Plus MasterMix | 5.0 ml (200 Rxns) | |
| G014-dye | 2X PCR Taq Plus MasterMix with dye | 5.0 ml (200 Rxns) | |
| G277 | TaqFast DNA Polymerase | 250 U (50 µl) | |
| G278 | TaqFast DNA Polymerase | 1000U(200 μl) | |
| G280 | 2X PCR TaqFast MasterMix | ||
| G280-dye | 2X PCR TaqFast MasterMix with dye | ||
| G078 | Precision ; DNA Polymerase | 500 U (100 µl) | |
| G011 | HotStart DNA Polymerase | 250 U (50 µl) | |
| G039 | HotStart DNA Polymerase | 1000U(200 µl) | |
| G010 | dNTP Mix | 250µl | |
| G128 | dNTP Mix | 500 µl | |
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文献和实验Long-Range PCR with a DNA Polymerase Fusion
Proofreading DNA polymerase fusions offer several advantages for long-range PCR, including faster run times and higher fidelity compared with Taq -based enzymes. However, their use so far has been limited to amplification of small
Conventional polymerase chain reaction (PCR) enables reliable amplification of 3–4 kb of DNA (1 ) while attempts at optimisation has enabled 15.6 kb of λ DNA to be amplified (2 ). The maximum amplifiable length of PCR is limited by the low
The heat-stable DNA polymerase utilized in the polymerase chain reaction (PCR) has been widely used to amplify DNA fragments since its conception by Kerry Mullis in 1985. However, it soon became apparent that there was a constraint
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