LinKine™ AbFluor™ 488偶联试剂盒

LinKine™ AbFluor™ 488偶联试剂盒（货号：KTL0520）

产品核心参数

• 产品分类：核心产品 - 蛋白偶联
• 应用领域：蛋白类生物分子标记，适配488 nm激光激发系统
• 规格选项：3×100 μg（¥1689）｜1 mg（¥2716）
• 交付保障：现货库存，支持次日速递

创新技术特性

• 预活化荧光染料：即用型AbFluor™ 488溶液，消除传统标记试剂需预活化的操作步骤
• 智能缓冲体系：专利配方缓冲液实现1:3-1:5染料/蛋白优化配比，荧光量子产率达0.92
• 一体化纯化方案：标配50KD分子筛纯化柱，15分钟完成游离染料清除，蛋白回收率＞85%

专业操作指南

组分名称	保存条件	功能说明
活化AbFluor™ 488	-20℃避光	即用型反应基团(-NHS ester)
偶联缓冲液	4℃	pH8.3磷酸盐体系，不含干扰性氨基
纯化柱(50KD)	常温	超滤分子量截留值50,000 Da

关键操作参数

• 标记样本浓度要求：≥2 mg/ml（推荐PBS体系）
• 温度敏感性：全程4℃操作，避光条件执行
• 缓冲液限制：严禁含Tris、甘氨酸等氨基成分

质量承诺

本产品经HPLC验证标记效率＞92%，批间差异＜5%，兼容FACS/IF/ICC等检测平台。
*产品研发用途专用，不适用于体外诊断或治疗应用

技术支持信息

• 冷链运输：全程蓝冰维持2-8℃
• 有效期：未开封组分-20℃保存6个月
• 替代方案：完美匹配Alexa Fluor 488/FITC光学通道

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注意：以上信息可能随着产品优化升级等因素产生变动，具体信息请咨询亚科因公司。

LinKine™ AbFluor™ 488 Conjugation Kit (Cat. No.: KTL0520)

Core Product Specifications

• Product Category: Core Products - Protein Conjugation
• Application: Biomolecular labeling of proteins, compatible with 488 nm laser excitation systems
• Specification Options: 3×100 μg (¥1689)｜1 mg (¥2716)
• Delivery Guarantee: In-stock, next-day delivery available

Innovative Technical Features

• Pre-activated Fluorescent Dye: Ready-to-use AbFluor™ 488 solution, eliminating pre-activation steps required by traditional labeling reagents
• Smart Buffer System: Patented buffer formulation achieves 1:3-1:5 optimal dye/protein ratio with fluorescence quantum yield of 0.92
• Integrated Purification Solution: Includes 50KD size-exclusion purification column for complete removal of free dyes within 15 minutes, with protein recovery rate >85%

Professional Operation Guide

Component	Storage Conditions	Functional Description
Activated AbFluor™ 488	-20℃, protect from light	Ready-to-use reactive group (-NHS ester)
Conjugation Buffer	4℃	Phosphate system (pH8.3), amine-free
Purification Column (50KD)	Room temperature	Ultrafiltration molecular weight cutoff: 50,000 Da

Critical Operation Parameters

• Sample concentration requirement: ≥2 mg/ml (recommended in PBS system)
• Temperature sensitivity: Operate at 4℃ under light-protected conditions
• Buffer restrictions: Strictly avoid amine-containing components (e.g., Tris, glycine)

Quality Commitment

This product has been HPLC-validated with >92% labeling efficiency and <5% batch-to-batch variation, compatible with FACS/IF/ICC detection platforms.
*For research use only. Not for diagnostic or therapeutic applications.

Technical Support Information

• Cold-chain transportation: Maintained at 2-8℃ with blue ice
• Shelf life: 6 months when stored unopened at -20℃
• Alternative options: Perfectly matches Alexa Fluor 488/FITC optical channels

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Note: The above information may be subject to changes due to product optimization and upgrades. Please consult ACROBiosystems for specific details.