- ￥2600
- 原装进口
- Range 31.25-2000 pg/mL
- KE00239
- KE00239
- 2025年10月16日
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- 详细信息
- 文献和实验
- 技术资料
- 标记物：
KE00239
- 库存：
1周
- 供应商：
上海研卉生物科技有限公司
- 应用：
Sensitivity 0.39 pg/mL
- 样本：
serum, plasma and other biological fluids.
- 适应物种：
人
- 检测方法：
elisa
- 检测范围：
Range 31.25-2000 pg/mL
- 规格：
96T
CD 蛋白(损坏性关节炎)（YKL-40）ELISA试剂盒详细说明
产品中文名称：CD 蛋白(损坏性关节炎)（YKL-40）ELISA试剂盒产品英文名称：YKL-40 ELISA Kit ELISA Kit
Synonyms
39 kDa synovial protein, ASRT7, Cartilage glycoprotein 39, CGP 39, CHI3L1, Chitinase 3 like protein 1, DKFZp686N19119, FLJ38139, GP 39, GP39, HC gp39, hCGP 39, HCGP 3P, YKL 40, YKL40, YYL 40
Product Information
KE00239 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The Human CHI3L1/YKL40 ELISA kit is to be used to detect and quantify protein levels of endogenous human CHI3L1/YKL40. The assay recognizes human CHI3L1/YKL40. An antibody specific for human CHI3L1/YKL40 has been pre-coated onto the microwells. The human CHI3L1/YKL40 protein in samples is captured by the coated antibody after incubation. Following extensive washing, another antibody of biotinylated specific forhuman CHI3L1/YKL40 is added to detect the captured human CHI3L1/YKL40 protein. For signal development, Streptavidin-HRP is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sul-furic acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Human serum | 89% | 81%-105% |
| Cell culture supernatants | 87% | 83%-93% |
| Human urine | 93% | 79%-102% |
IntraAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 20 | 989.82 | 24.78 | 2.5 |
| 2 | 20 | 239.03 | 8.27 | 3.5 |
| 3 | 20 | 67.01 | 2.07 | 3.1 |
InterAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 24 | 1,086.40 | 20.08 | 1.9 |
| 2 | 24 | 279.98 | 5.31 | 1.9 |
| 3 | 24 | 71.30 | 3.63 | 5.1 |
Background Information
Chitinase 3-like-1 (CHI3L1/YKL-40/CGP-39/GP-39/hCGP-39) is a protein secreted from restricted cell types including colonic epithelial cells (CECs) and macrophages. CHI3L1 is an inflammation-associated molecule, and its expression is enhanced in persons with colitis and colon cancer. CHI3L1 is a 40 kDa protein and is produced by restricted cell types, including colonic epithelial cells (CECs) and macrophages. CHI3L1 can be detected in the Golgi apparatus and the endoplasmic reticulum, but its major sites of action seems to be extracellular as a secreted protein.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | 39 kDa synovial protein, ASRT7, Cartilage glycoprotein 39, CGP 39, CHI3L1, Chitinase 3 like protein 1, DKFZp686N19119, FLJ38139, GP 39, GP39, HC gp39, hCGP 39, HCGP 3P, YKL 40, YKL40, YYL 40 |
| Product name | Human CHI3L1/YKL40 ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Serum, Plasma, Cell culture supernatants , Urine |
| Assay type | Sandwich |
| Sensitivity | 0.39 pg/mL |
| Range | 31.25-2000 pg/mL |
| Reactivity | Human |
| Tested applications | Sandwich ELISA |
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文献和实验人CD蛋白(损坏性关节炎)（YKL-40） 酶联免疫 分析（ ELISA ） 试剂 盒使用说明书 本试剂仅供研究使用 目的：本试剂盒用于测定人血清，血浆及相关液体样本中CD蛋白(损坏性关节炎)（YKL-40）含量。 实验原理 ： 本试剂盒应用双抗体夹心法测定 标本 中 人 CD蛋白 ( 损坏性关节炎 ) （ YKL-40 ） 水平。用纯化的 人CD 蛋白 ( 损坏性关节炎 ) （ YKL-40 ） 抗体包被微孔
葡萄糖来源 AGEs ELISA 检测试剂盒 （ Glucose-derived AGEs ELISA Kit ） 研 究 背 景 越来越多的证据指出晚期糖基化终端产物（ AGEs ）修饰蛋白严重影响到老龄化人群的健康问题。 AGEs 是葡萄糖与自由的蛋白质氨基基团发生非酶促糖基化反应的终端不可逆代谢产物的总称。其在人体内的积累会引发组织或器官一系列的病理性变化，从而导致白内障、阿尔茨海默氏病、骨关节炎、心功
的诊疗重点是尽早诊断出类风关患者，积极采用干预治疗。 鉴于此，寻找高敏感性高特异性的RA早期检测指标便成为近年来类风湿关节炎研究的热点。 这一诊断指标——抗CCP抗体，终于在2000年诞生，并于2009年ACR会议上得到公认，被列为RA早期诊断的血清学指标之一。其高敏感性和极高的 特异性，且阳性提示患者易出现关节骨质损坏的预后性得到广泛的认可，在欧美国家被列入RA的诊断标准中，作为常规项目检测，并广泛进入体检检项。 上海富莼科芯生物技术股份有限公司于2006年7月在中国首次推出国产第二代抗
