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- 详细信息
- 文献和实验
- 技术资料
- 免疫原:
Recombinant human C5, C5a protein
- 亚型:
IgG2a
- 保存条件:
Store at -20°C. Stable for one year after shipment.
- 克隆性:
Monoclonal
- 标记物:
Unconjugated
- 适应物种:
human
- 保质期:
详见产品包装
- 库存:
充足
- 宿主:
Mouse
- 应用范围:
WB, IF/ICC, FC (Intra), ELISA
- 靶点:
C5
- 抗体英文名:
C5 Monoclonal antibody
- 抗体名:
C5 Monoclonal antibody
- 规格:
50ul/100ul/150ul
| 规格: | 50ul | 产品价格: | ¥1350.0 |
|---|---|---|---|
| 规格: | 100ul | 产品价格: | ¥2200.0 |
| 规格: | 150ul | 产品价格: | ¥3000.0 |
经过测试的应用
| Positive WB detected in | human plasma tissue, rabbit serum |
| Positive IF/ICC detected in | HepG2 cells |
| Positive FC (Intra) detected in | HepG2 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:400-1:1600 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
66634-1-Ig targets C5 in WB, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, IF/ICC, FC (Intra), ELISA |
| 文献引用应用 | WB, ELISA |
| 经测试反应性 | human |
| 文献引用反应性 | human |
| 免疫原 |
CatNo: Ag17882 Product name: Recombinant human C5, C5a protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 1418-1676 aa of BC113740 Sequence: GSSHAVMDISLPTGISANEEDLKALVEGVDQLFTDYQIKDGHVILQLNSIPSSDFLCVRFRIFELFEVGFLSPATFTVYEYHRPDKQCTMFYSTSNIKIQKVCEGAACKCVEADCGQMQEELDLTISAETRKQTACKPEIAYAYKVSITSITVENVFVKYKATLLDIYKTGEAVAEKDSEITFIKKVTCTNAELVKGRQYLIMGKEALQIKYNFSFRYIYPLDSLTWIEYWPRDTTCSSCQAFLANLDEFAEDIFLNGC |
| 宿主/亚型 | Mouse / IgG2a |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | complement component 5 |
| 别名 | C5, C5a, C5, C5b, 2G8C9, C3 and PZP-like alpha-2-macroglobulin domain-containing protein 4, C5a anaphylatoxin |
| 计算分子量 | 1676 aa, 188 kDa |
| 观测分子量 | 115 kDa |
| GenBank蛋白编号 | BC113740 |
| 基因名称 | C5 |
| Gene ID (NCBI) | 727 |
| RRID | AB_2881993 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | P01031 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
The complement system is an important effector that bridges the innate and adaptive immune systems. The fifth component of complement, C5, is part of the complement cascade and plays an important role in inflammation and cell killing. It consists of disulfide-linked alpha and beta polypeptide chains but can be cleaved into two active peptides (C5a and C5b) by C5 convertases. Mutations and defects in C5 are associated with severe recurrent infections. (PMID: 20010915; PMID: 8011297; PMID: 6554279)
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文献和实验| Species | Application | Title |
|---|---|---|
| human | WB | Mol Cell Proteomics Proteomic landscape of exosomes reveals the functional contributions of CD151 in triple-negative breast cancer.Authors - Sipeng Li |
| human | WB | Sci Rep Natural antibodies and CRP drive anaphylatoxin production by urate crystals.Authors - Anne Kathrin Wessig |
| ELISA | Virulence Streptococcus suis subtilisin-like serine proteases SspA-1 and SspA-2 interplay with complement C3a and C5a to facilitate bacterial immune evasion and infectionAuthors - Simin Deng |
的分子,如CD2、CD4、CD8、CD28、CD31、CD50、CD54、CD56、CD58、CD80、CD102、CD106等。 (5)有CD命名的CR有CR1(CD35)、CR2(CD21)、CR3(CD11b\CD18)、CR4(CD11c/CD18)和C5aR(CD88),其中CR3和CR4属于粘附分子中整合素超家族成员。 (6)有CD编号的CKR有CD115~CDw130;属于IGSF结构的CKR有CD115、CD
一、实验原理 将抗C5a单克隆抗体包被固相聚苯乙烯板,加入经聚乙二醇(PEG)处理的待测样本,然后依次加入兔抗人C5a-IgC多克隆抗体和过氧化物酶标记的猪抗兔IsC,再加入底物2,2’-连氮-双(3-乙基苯骈唑啉-6-磺酸盐)(ABTS)和H2O2显色,于酶标仪405 mn处测定每分钟光密度增加量(OD/min),以OD/min为纵坐标,C5a标准晶浓度为横坐标绘制标准曲线图,求出待测样本中C5a的含量。 二、实验试剂 1. PBSPH7.2
抗体的检测外,还应该用与其抗原成分相关的其它抗原进行交叉试验,方法可用ELISA、IFA法。例如:①制备抗黑色素瘤细胞的McAb,除用黑色素瘤细胞反应外,还应该用其它脏器的肿瘤细胞和正常细胞进行交叉反应,以便挑选肿瘤特异性或肿瘤相关抗原的单克隆抗体。②制备抗重组的细胞因子的单克隆抗体,应首先考虑是否与表达菌株的蛋白有交叉反应,其次是与其它细胞因子间有无交叉。 2.McAb的Ig类与亚类的鉴定 一般在用酶标或荧光素标记的第二抗体进行筛选时已经基本上确定了抗体的Ig类型
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