- ￥1350 - 3000
- Proteintech
- 66294-1-Ig
- 2025年10月13日
- IHC, IF-P, ELISA
- Mouse
- human
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- 详细信息
- 询价记录
- 文献和实验
- 技术资料
- 免疫原：
Recombinant human VCAM-1 protein
- 亚型：
IgG2a
- 保存条件：
Store at -20°C. Stable for one year after shipment.
- 克隆性：
Monoclonal
- 标记物：
Unconjugated
- 适应物种：
human
- 保质期：
详见产品包装
- 库存：
充足
- 宿主：
Mouse
- 应用范围：
IHC, IF-P, ELISA
- 靶点：
VCAM-1/CD106
- 抗体英文名：
VCAM-1/CD106 Monoclonal antibody
- 抗体名：
VCAM-1/CD106 Monoclonal antibody
- 规格：
50ul/100ul/150ul
| 规格： | 50ul | 产品价格： | ￥1350.0 |
|---|---|---|---|
| 规格： | 100ul | 产品价格： | ￥2200.0 |
| 规格： | 150ul | 产品价格： | ￥3000.0 |
经过测试的应用
| Positive IHC detected in | human tonsillitis tissue, human lung cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF-P detected in | human tonsillitis tissue |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Immunohistochemistry (IHC) | IHC : 1:2000-1:8000 |
| Immunofluorescence (IF)-P | IF-P : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
66294-1-Ig targets VCAM-1/CD106 in IHC, IF-P, ELISA applications and shows reactivity with human samples.
| 经测试应用 | IHC, IF-P, ELISA |
| 文献引用应用 | IHC, IF |
| 经测试反应性 | human |
| 文献引用反应性 | human, rat |
| 免疫原 |
CatNo: Ag24697 Product name: Recombinant human VCAM-1 protein Source: e coli.-derived, PET30a Tag: 6*His Domain: 341-739 aa of BC017276 Sequence: ESPSFSWRTQIDSPLSGKVRSEGTNSTLTLSPVSFENEHSYLCTVTCGHKKLEKGIQVELYSFPRDPEIEMSGGLVNGSSVTVSCKVPSVYPLDRLEIELLKGETILENIEFLEDTDMKSLENKSLEMTFIPTIEDTGKALVCQAKLHIDDMEFEPKQRQSTQTLYVNVAPRDTTVLVSPSSILEEGSSVNMTCLSQGFPAPKILWSRQLPNGELQPLSENATLTLISTKMEDSGVYLCEGINQAGRSRKEVELIIQVTPKDIKLTAFPSESVKEGDTVIISCTCGNVPETWIILKKKAETGDTVLKSIDGAYTIRKAQLKDAGVYECESKNKVGSQLRSLTLDVQGRENNKDYFSPELLVLYFASSLIIPAIGMIIYFARKANMKGSYSLVEAQKSKV |
| 宿主/亚型 | Mouse / IgG2a |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | vascular cell adhesion molecule 1 |
| 别名 | VCAM1, VCAM-1, Soluble Vascular Cell Adhesion Molecule-1, L1CAM, INCAM-100 |
| 计算分子量 | 739 aa, 81 kDa |
| 观测分子量 | 110 kDa |
| GenBank蛋白编号 | BC017276 |
| 基因名称 | VCAM-1 |
| Gene ID (NCBI) | 7412 |
| RRID | AB_2881677 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | P19320 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Vascular cell adhesion molecule 1 (VCAM1), also known as CD106, is a 110-kDa transmembrane glycoprotein belonging to the immunoglobulin gene superfamily. VCAM1 is expressed by cytokine-activated endothelium, interacts with integrin VLA4 (α4β1) present on the surface of leukocytes, and mediates both adhesion and signal transduction. It is also expressed either constitutively or inducibly in a variety of other cell types, including vascular smooth muscle cells, differentiating skeletal muscle cells, renal and neural epithelial cells, macrophages (Kupffer cells), dendritic cells, and bone marrow stromal cells (PMID: 7507076, 11359843).
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| human | Int J Biol Macromol Novel soybean polypeptide dglycin alleviates atherosclerosis in apolipoprotein E-deficient miceAuthors - Han Zhao | |
| human | Life Sci PMID: 29452166Authors - Qiangbing Yang | |
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部分（α3），Poly IgR与多聚Ig，FcγRⅠ（CD64）、FcγRⅡ(CD32)、FcγRⅢ（CD16）与Ig Fc段，FcεRⅠ与IgE Fc段，FcαR（CD89）与IgA Fc段，CD28与B7/BB1（CD80）等之间的相互识别。 2.IGSF和integrin相互识别 如ICAM-1（CD54）、ICAM-2（CD102）、ICAM-3（CD50）与LFA-1（CD11a/CD18），VCAM-1（CD106）与VLA-4（CD49d/CD29)之间的相互作用。
抗体的检测外，还应该用与其抗原成分相关的其它抗原进行交叉试验，方法可用ELISA、IFA法。例如：①制备抗黑色素瘤细胞的McAb，除用黑色素瘤细胞反应外，还应该用其它脏器的肿瘤细胞和正常细胞进行交叉反应，以便挑选肿瘤特异性或肿瘤相关抗原的单克隆抗体。②制备抗重组的细胞因子的单克隆抗体，应首先考虑是否与表达菌株的蛋白有交叉反应，其次是与其它细胞因子间有无交叉。 2．McAb的Ig类与亚类的鉴定 一般在用酶标或荧光素标记的第二抗体进行筛选时已经基本上确定了抗体的Ig类型
如ICAM-1（CD54）、ICAM-2（CD102）、ICAM-3（CD50）与LFA-1（CD11a/CD18），VCAM-1（CD106）与VLA-4（CD49d/CD29)之间的相互作用。 3.IGSF和其它分子的相互识别 包括TCR识别MHCⅠ类或Ⅱ类分子与抗原复全物，细胞因子受体识别细胞因子等。 (责任编辑：大汉昆仑王)
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