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- 详细信息
- 文献和实验
- 技术资料
- 免疫原:
Recombinant human IL-22RA2 protein
- 亚型:
IgG2a
- 保存条件:
Store at -20°C. Stable for one year after shipment.
- 克隆性:
Monoclonal
- 标记物:
Unconjugated
- 适应物种:
human
- 保质期:
详见产品包装
- 库存:
充足
- 宿主:
Mouse
- 应用范围:
WB, IHC, IF-P, ELISA
- 靶点:
IL-22RA2
- 抗体英文名:
IL-22RA2 Monoclonal antibody
- 抗体名:
IL-22RA2 Monoclonal antibody
- 规格:
50ul/100ul/150ul
| 规格: | 50ul | 产品价格: | ¥1350.0 |
|---|---|---|---|
| 规格: | 100ul | 产品价格: | ¥2200.0 |
| 规格: | 150ul | 产品价格: | ¥3000.0 |
经过测试的应用
| Positive WB detected in | Raji cells |
| Positive IHC detected in | mouse kidney tissue, human breast cancer tissue, human lung cancer tissue, human small intestine tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF-P detected in | human breast cancer tissue |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
| Immunofluorescence (IF)-P | IF-P : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
66190-1-Ig targets IL-22RA2 in WB, IHC, IF-P, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, IHC, IF-P, ELISA |
| 文献引用应用 | WB, IHC, IF |
| 经测试反应性 | human |
| 文献引用反应性 | human, pig |
| 免疫原 |
CatNo: Ag18682 Product name: Recombinant human IL-22RA2 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 1-232 aa of BC125167 Sequence: MMPKHCFLGFLISFFLTGVAGTQSTHESLKPQRVQFQSRNFHNILQWQPGRALTGNSSVYFVQYKIYGQRQWKNKEDCWGTQELSCDLTSETSDIQEPYYGRVRAASAGSYSEWSMTPRFTPWWETKIDPPVMNITQVNGSLLVILHAPNLPYRYQKEKNVSIEDYYELLYRVFIINNSLEKEQKVYEGAHRAVEIEALTPHSSYCVVAEIYQPMLDRRSQRSEERCVEIP |
| 宿主/亚型 | Mouse / IgG2a |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | interleukin 22 receptor, alpha 2 |
| 别名 | IL22RA2, CRF2 10, CRF2 S1, CRF2-10, CRF2-S1 |
| 计算分子量 | 263 aa, 31 kDa |
| 观测分子量 | 35 kDa |
| GenBank蛋白编号 | BC125167 |
| 基因名称 | IL-22RA2 |
| Gene ID (NCBI) | 116379 |
| RRID | AB_2881585 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | Q969J5 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Interleukin-22 receptor subunit alpha-2 (IL22RA2) is a soluble member of the type II cytokine receptor family. Through binding to IL22, IL22RA2 prevents the interaction of IL22 with the functional IL22-R complex and blocks the activity of IL22 (in vitro). IL22RA2 may play an important role as an IL22 antagonist in the regulation of inflammatory responses. Alternative splicing results in multiple transcript variants encoding distinct isoforms.
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文献和实验| Species | Application | Title |
|---|---|---|
| pig | WB,IHC | Front Cell Infect Microbiol Oral Administration of Lactobacillus rhamnosus GG Ameliorates Salmonella Infantis-Induced Inflammation in a Pig Model via Activation of the IL-22BP/IL-22/STAT3 Pathway.Authors - Gui-Yan Yang |
Acta Pharmacol Sin FSHR ablation induces depression-like behaviors.Authors - Wen-Kai Bi | ||
| human | Cells The Rare IL22RA2 Signal Peptide Coding Variant rs28385692 Decreases Secretion of IL-22BP Isoform-1, -2 and -3 and Is Associated with Risk for Multiple Sclerosis.Authors - Paloma Gómez-Fernández | |
| human | WB,IF | Front Immunol Long Interleukin-22 Binding Protein Isoform-1 Is an Intracellular Activator of the Unfolded Protein Response.Authors - Paloma Gómez-Fernández |
抗体的检测外,还应该用与其抗原成分相关的其它抗原进行交叉试验,方法可用ELISA、IFA法。例如:①制备抗黑色素瘤细胞的McAb,除用黑色素瘤细胞反应外,还应该用其它脏器的肿瘤细胞和正常细胞进行交叉反应,以便挑选肿瘤特异性或肿瘤相关抗原的单克隆抗体。②制备抗重组的细胞因子的单克隆抗体,应首先考虑是否与表达菌株的蛋白有交叉反应,其次是与其它细胞因子间有无交叉。 2.McAb的Ig类与亚类的鉴定 一般在用酶标或荧光素标记的第二抗体进行筛选时已经基本上确定了抗体的Ig类型
-CAM-L1、MAG、Po、F3、NILE、TAG1等以及CD22、CD48、α1-BgP分子等。某此IgH链中C H 2和C H 3可有8个β折叠,在这种情况下,其中一个β折叠股只有3个氨基酸残基。 在V、C1和C2三组中,有些Ig超家族成员之间的同源性要大于该组中平均的类似程度,如在V组中IgV、TCR-V、和CD8V结构域;C1组中IgC、TCR-C、MHC-C结构域;C2组中CD2和LFA-3,NCAM、NCAM-L1和MAG
免疫系统受抗原刺激后,B细胞转化为浆细胞,由浆细胞产生与抗原发生特异性结合的球蛋白,这类球蛋白称为抗体(antibody,Ab)。 免疫球蛋白(immunoglobulim,Ig)是指具有抗体活性或化学结构与抗体相似的球蛋白。 免疫球蛋白普遍存在于哺乳动物和人类的血液、组织液和外分泌中。人体的免疫球蛋白主要有五类:IgM、IgA、IgG、IgD、IgE。 (一) 抗体结构 抗体是具有4条多肽链的对称
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