- ￥1350 - 3000
- Proteintech
- 66082-1-Ig
- 2025年10月13日
- WB, IHC, IF/ICC, ELISA
- Mouse
- human, zebrafish, rat, mouse
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- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
None
- 亚型：
IgG2b
- 保存条件：
Store at -20°C. Stable for one year after shipment.
- 克隆性：
Monoclonal
- 标记物：
Unconjugated
- 适应物种：
human, zebrafish, rat, mouse
- 保质期：
详见产品包装
- 库存：
充足
- 宿主：
Mouse
- 应用范围：
WB, IHC, IF/ICC, ELISA
- 靶点：
CISD2-Specific
- 抗体英文名：
CISD2-Specific Monoclonal antibody
- 抗体名：
CISD2-Specific Monoclonal antibody
- 规格：
50ul/100ul/150ul
| 规格： | 50ul | 产品价格： | ￥1350.0 |
|---|---|---|---|
| 规格： | 100ul | 产品价格： | ￥2200.0 |
| 规格： | 150ul | 产品价格： | ￥3000.0 |
经过测试的应用
| Positive WB detected in | fetal human brain tissue, MCF-7 cells, HeLa cells, ROS1728 cells, RAW 264.7 cells, zebrafish tissue |
| Positive IHC detected in | human kidney tissue, human testis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | MCF-7 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:16000 |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
66082-1-Ig targets CISD2-Specific in WB, IHC, IF/ICC, CoIP, ELISA applications and shows reactivity with human, zebrafish, rat, mouse samples.
| 经测试应用 | WB, IHC, IF/ICC, ELISA |
| 文献引用应用 | WB, CoIP |
| 经测试反应性 | human, zebrafish, rat, mouse |
| 文献引用反应性 | human, mouse |
| 免疫原 |
fusion protein |
| 宿主/亚型 | Mouse / IgG2b |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | CDGSH iron sulfur domain 2 |
| 别名 | CDGSH iron sulfur domain 2, CDGSH2, CISD2, CISD2-Specific, ERIS, Miner1, MitoNEET related 1 protein, NAF 1, WFS2, ZCD2 |
| 观测分子量 | 15 kDa |
| GenBank蛋白编号 | NM_001008388 |
| 基因名称 | CISD2 |
| Gene ID (NCBI) | 493856 |
| RRID | AB_11232425 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | Q8N5K1 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
CISD2 gene encodes a 15 kDa CDGSH iron-sulfur domain-containing protein 2, which is also named Miner1 or NAF-1, this protein was reported on endoplasmic reticulum membrane or mitochondrion outer membrane. Defects in CISD2 are the cause of Wolfram syndrome type 2 (WFS2), a rare disorder characterized by juvenile-onset insulin-dependent diabetes mellitus with optic atrophy. CISD2 regulates autophagy program by interacting BCL2, contributing to antagonize BECN1-mediated cellular autophagy at the endoplasmic reticulum. This monoclonal antibody is specific to CISD2 and does not cross-react with CISD1.
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文献和实验| Species | Application | Title |
|---|---|---|
| human | WB | Commun Biol Integrating apaQTL and eQTL analysis identifies a potential causal variant associated with lung adenocarcinoma risk in the Chinese populationAuthors - Huiwen Xu |
| human | WB | J Biomed Sci Extracellular arginine availability modulates eIF2α O-GlcNAcylation and heme oxygenase 1 translation for cellular homeostasisAuthors - Yu-Wen Hung |
Mol Neurodegener Mitochondrial CISD1/Cisd accumulation blocks mitophagy and genetic or pharmacological inhibition rescues neurodegenerative phenotypes in Pink1/parkin modelsAuthors - Aitor Martinez | ||
| human,mouse | WB,CoIP | Nat Commun ER calcium depletion as a key driver for impaired ER-to-mitochondria calcium transfer and mitochondrial dysfunction in Wolfram syndromeAuthors - Mailis Liiv |
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抗体的检测外，还应该用与其抗原成分相关的其它抗原进行交叉试验，方法可用ELISA、IFA法。例如：①制备抗黑色素瘤细胞的McAb，除用黑色素瘤细胞反应外，还应该用其它脏器的肿瘤细胞和正常细胞进行交叉反应，以便挑选肿瘤特异性或肿瘤相关抗原的单克隆抗体。②制备抗重组的细胞因子的单克隆抗体，应首先考虑是否与表达菌株的蛋白有交叉反应，其次是与其它细胞因子间有无交叉。 2．McAb的Ig类与亚类的鉴定 一般在用酶标或荧光素标记的第二抗体进行筛选时已经基本上确定了抗体的Ig类型
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