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- 详细信息
- 文献和实验
- 技术资料
- 免疫原:
None
- 亚型:
IgG
- 保存条件:
Store at -20°C.
- 克隆性:
Polyclonal
- 标记物:
Unconjugated
- 适应物种:
human
- 保质期:
详见产品包装
- 库存:
充足
- 宿主:
Rabbit
- 应用范围:
IHC, IF/ICC, ELISA
- 靶点:
ESR2 Beta 3
- 抗体英文名:
ESR2 Beta 3 Polyclonal antibody
- 抗体名:
ESR2 Beta 3 Polyclonal antibody
- 规格:
50ul/100ul/150ul
| 规格: | 50ul | 产品价格: | ¥1350.0 |
|---|---|---|---|
| 规格: | 100ul | 产品价格: | ¥2200.0 |
| 规格: | 150ul | 产品价格: | ¥3000.0 |
经过测试的应用
| Positive IHC detected in | human cervix tissue, human breast cancer tissue, human endometrial cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HeLa cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
55441-1-AP targets ESR2 Beta 3 in IHC, IF/ICC, ELISA applications and shows reactivity with human samples.
| 经测试应用 | IHC, IF/ICC, ELISA |
| 经测试反应性 | human |
| 免疫原 |
fusion protein |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | estrogen receptor 2 (ER beta) |
| 别名 | ER BETA, Erb, ESR BETA, ESR2, ESR2 Beta 3, ESRB, ESTRB, estrogen receptor 2 (ER beta), Estrogen receptor beta, NR3A2 |
| 计算分子量 | 58 kDa |
| GenBank蛋白编号 | NM_001437 |
| 基因名称 | ESR2 |
| Gene ID (NCBI) | 2100 |
| RRID | AB_2881334 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q92731 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Estrogen receptor-beta (ESR2) is a member of the superfamily of nuclear receptors, which can transduce extracellular signals into transcriptional responses. It binds estrogens with an affinity similar to that of ESR1, and activates expression of reporter genes containing estrogen response elements (ERE) in an estrogen-dependent manner. DNA-binding by ESR1 and ESR2 is rapidly lost at 37 degrees Celsius in the absence of ligand while in the presence of 17 beta-estradiol and 4-hydroxy-tamoxifen loss in DNA-binding at elevated temperature is more gradual
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文献和实验10X TBS:24.2 g Trisbase, 80 g NaCl;用1N HCl调pH为 7.6 7. 甲醇 8. TBS/T缓冲液:1X TBS, 0.1% Tween-20 9. 封闭缓冲液(TBS/T):1X TBS,0.1% Tween-20加5% w/v脱脂奶粉或BSA 10. 一抗的稀释:1X TBS,0.1% Tween-20 加 5% BSA ( 多抗) 或 5%脱脂奶粉( 单抗)。Note: 一般来说,BSA被推荐用于多克隆抗体
10X TBS:24.2 g Trisbase, 80 g NaCl;用1N HCl调pH为 7.6 7. 甲醇 8. TBS/T缓冲液:1X TBS, 0.1% Tween-20 9. 封闭缓冲液(TBS/T):1X TBS,0.1% Tween-20加5% w/v脱脂奶粉或BSA 10. 一抗的稀释:1X TBS,0.1% Tween-20 加 5% BSA ( 多抗) 或 5%脱脂奶粉( 单抗)。Note: 一般来说,BSA被推荐用于多克隆抗体
7. 甲醇 8. TBS/T缓冲液:1X TBS, 0.1% Tween-20 9. 封闭缓冲液(TBS/T):1X TBS,0.1% Tween-20加5% w/v脱脂奶粉或BSA 10. 一抗的稀释:1X TBS,0.1% Tween-20 加 5% BSA ( 多抗) 或 5%脱脂奶粉( 单抗)。Note: 一般来说,BSA被推荐用于多克隆抗体,脱脂奶粉用于单克隆抗体,这样可得到较高的信噪比。预染的蛋白质Marker,可用于监测转膜的效率。 样品制备 原始样品可为细胞、组织
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