- ￥1350 - 3000
- Proteintech
- 51066-1-AP
- 2025年10月13日
- WB, IP, IHC, ELISA
- Rabbit
- human, mouse
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- 详细信息
- 询价记录
- 文献和实验
- 技术资料
- 免疫原：
None
- 亚型：
IgG
- 保存条件：
Store at -20°C.
- 克隆性：
Polyclonal
- 标记物：
Unconjugated
- 适应物种：
human, mouse
- 保质期：
详见产品包装
- 库存：
充足
- 宿主：
Rabbit
- 应用范围：
WB, IP, IHC, ELISA
- 靶点：
IkB Alpha
- 抗体英文名：
IkB Alpha Polyclonal antibody
- 抗体名：
IkB Alpha Polyclonal antibody
- 规格：
50ul/100ul/150ul
| 规格： | 50ul | 产品价格： | ￥1350.0 |
|---|---|---|---|
| 规格： | 100ul | 产品价格： | ￥2200.0 |
| 规格： | 150ul | 产品价格： | ￥3000.0 |
经过测试的应用
| Positive WB detected in | PC-3 cells, HeLa cells, Jurkat cells |
| Positive IP detected in | HeLa cells |
| Positive IHC detected in | human cervical cancer tissue, human liver tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
51066-1-AP targets IkB Alpha in WB, IHC, IP, ELISA applications and shows reactivity with human, mouse samples.
| 经测试应用 | WB, IP, IHC, ELISA |
| 文献引用应用 | WB |
| 经测试反应性 | human, mouse |
| 文献引用反应性 | human, mouse, rat |
| 免疫原 |
fusion protein |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor, alpha |
| 别名 | I kappa B alpha, IkappaBalpha, IkB alpha, IKBA, MAD 3, MAD3, NF kappa B inhibitor alpha, NFKBI, NFKBIA |
| 计算分子量 | 36 kDa |
| 观测分子量 | 39 kDa |
| GenBank蛋白编号 | BC002601 |
| 基因名称 | IkB Alpha |
| Gene ID (NCBI) | 4792 |
| RRID | AB_2235946 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | P25963 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor, alpha (NFKBIA, synonyms: IKBA, MAD-3, NFKBI). NFKB1 or NFKB2 is bound to REL, RELA , or RELB to form the NFKB complex. The NFKB complex is inhibited by I-kappa-B proteins (NFKBIA or NFKBIB), which inactivate NF-kappa-B by trapping it in the cytoplasm. Phosphorylation of serine residues on the I-kappa-B proteins by kinases (IKBKA, or IKBKB) marks them for destruction via the ubiquitination pathway, thereby allowing activation of the NF-kappa-B complex. Activated NFKB complex translocates into the nucleus and binds DNA at kappa-B-binding motifs such as 5-prime GGGRNNYYCC 3-prime or 5-prime HGGARNYYCC 3-prime.
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- 作者
- 内容
- 询问日期
文献和实验| Species | Application | Title |
|---|---|---|
| WB | J Hazard Mater Melatonin alleviates Ochratoxin A-induced liver inflammation involved intestinal microbiota homeostasis and microbiota-independent manner.Authors - Daiyang Xia | |
| human | WB | J Immunol Enterovirus 71 2C protein inhibits TNF-α-mediated activation of NF-κB by suppressing IκB kinase β phosphorylation.Authors - Zheng Zhenhua Z |
| mouse | WB | Front Immunol Aspartate Metabolism Facilitates IL-1β Production in Inflammatory Macrophages.Authors - Hao Wang |
| mouse | WB | Front Immunol Serine Supports IL-1β Production in Macrophages Through mTOR Signaling.Authors - Siyuan Chen |
| mouse | WB | FASEB J Nintedanib ameliorates osteoarthritis in mice by inhibiting synovial inflammation and fibrosis caused by M1 polarization of synovial macrophages via the MAPK/PI3K-AKT pathwayAuthors - Jiangbo Yan |
| mouse | WB | Nutrients Fish Hydrolysate Supplementation Containing n-3 Long Chain Polyunsaturated Fatty Acids and Peptides Prevents LPS-Induced Neuroinflammation.Authors - Mathilde Chataigner |
【求助】矛盾的实验结果：IKb alpha和NFKB同时升高
吴佰霖 我的基因过表达，结合文献，推理应该是IKb alpha下降，而NFKB升高 （IKb可磷酸化NFKB，使后者降低） 但现在我的结果是两者同时升高，请问可能的原因有哪些呢？ 谢谢 woxingwosu 可否检测一下NFKB的磷酸化情况？ erik NFKB是检测核内的吗？检测p65还是p50/ 吴佰霖 检测总蛋白
细胞浆和细胞核蛋白，感觉还行，至少不影响实验结果。如果，你想精确的彻底的分离，或者想得到Oct-1的话，建议你采用蔗糖梯度离心方法再次富集你收集的各个组分上清，这可以分离得到比较纯的你想要检测的那些蛋白组分。楼主问：感谢指点，我有如下几个问题，多谢回答1、在实验过程中，我收集上清的时候的确是收集一部分，但是剩下的没有弃下，下次改进。2、对于我的抗体。Ikb-α是多克隆抗体，非特异性很强，感觉十分难做；OCT-1虽然是单抗，但我基本上没有检测它应该的大小（90K左右），只是检测到其它的分子量，不清
accumulation by LPS, as TLR4 can use either MyD88 or TRIF. The net result of these interactions was activation of the transcription factors NF-{kappa}B and AP-1, and synthesis of TNF-{alpha} mRNA. TNF-{alpha} acts through an apparent autocrine pathway
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