- ￥1350 - 3000
- Proteintech
- 23185-1-AP
- 2025年10月13日
- WB, IF/ICC, ELISA
- Rabbit
- human, mouse
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- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
Recombinant human P4HA3 protein
- 亚型：
IgG
- 保存条件：
Store at -20°C. Stable for one year after shipment.
- 克隆性：
Polyclonal
- 标记物：
Unconjugated
- 适应物种：
human, mouse
- 保质期：
详见产品包装
- 库存：
充足
- 宿主：
Rabbit
- 应用范围：
WB, IF/ICC, ELISA
- 靶点：
P4HA3
- 抗体英文名：
P4HA3 Polyclonal antibody
- 抗体名：
P4HA3 Polyclonal antibody
- 规格：
50ul/100ul/150ul
| 规格： | 50ul | 产品价格： | ￥1350.0 |
|---|---|---|---|
| 规格： | 100ul | 产品价格： | ￥2200.0 |
| 规格： | 150ul | 产品价格： | ￥3000.0 |
经过测试的应用
| Positive WB detected in | mouse kidney tissue, A549 cells |
| Positive IF/ICC detected in | A549 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
23185-1-AP targets P4HA3 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.
| 经测试应用 | WB, IF/ICC, ELISA |
| 文献引用应用 | WB, IHC |
| 经测试反应性 | human, mouse |
| 文献引用反应性 | human, mouse, rat |
| 免疫原 |
CatNo: Ag19716 Product name: Recombinant human P4HA3 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 28-256 aa of BC126170 Sequence: GDTFSALTSVARALAPERRLLGLLRRYLRGEEARLRDLTRFYDKVLSLHEDSTTPVANPLLAFTLIKRLQSDWRNVVHSLEASENIRALKDGYEKVEQDLPAFEDLEGAARALMRLQDVYMLNVKGLARGVFQRVTGSAITDLYSPKRLFSLTGDDCFQVGKVAYDMGDYYHAIPWLEEAVSLFRGSYGEWKTEDEASLEDALDHLAFAYFRAGNVSCALSLSREFLLY |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | prolyl 4-hydroxylase, alpha polypeptide III |
| 别名 | 4 PH alpha 3, P4HA3 |
| 计算分子量 | 544 aa, 61 kDa |
| 观测分子量 | 30 kDa, 61 kDa |
| GenBank蛋白编号 | BC126170 |
| 基因名称 | P4HA3 |
| Gene ID (NCBI) | 283208 |
| RRID | AB_2879228 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q7Z4N8 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Collagen prolyl 4-hydroxylases catalyze the hydroxylation of proline in x-pro-gly triplets in collagens and in proteins with collagen-like sequences. Collagen prolyl 4-hydroxylases are tetramers of 2 alpha subunits, such as P4HA3, and 2 beta subunits(P4HB). P4HA3 has 3 isoforms produced by alternative splicing with the MW of 61 kDa, 29 kDa and 67 kDa.
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文献和实验| Species | Application | Title |
|---|---|---|
| mouse,rat | WB | J Mol Cell Cardiol Ascorbate starvation alters endoplasmic reticulum-resident enzymes in cardiac fibroblasts, priming them for increased procollagen secretion.Authors - Randy T Cowling |
| human | WB | Commun Biol TGF-β-dependent reprogramming of amino acid metabolism induces epithelial-mesenchymal transition in non-small cell lung cancers.Authors - Fumie Nakasuka
|
| mouse | IHC | J Dent Sci Thrombospondin-1 and prolyl 4-hydroxylase subunit alpha 3 as potential biomarkers of salivary gland fibrosisAuthors - Zijian Zhang |
| human | WB | bioRxiv Identifying and targeting key driver genes for collagen production within the 11q13/14 breast cancer ampliconAuthors - Daniela Araiza-Olivera
|
| human | IHC | Heliyon Collagen synthase P4HA3 as a novel biomarker for colorectal cancer correlates with prognosis and immune infiltrationAuthors - Xiaohuan Guo |
| mouse | WB | Commun Biol Protein disulfide isomerase is essential for osteoblast differentiation in miceAuthors - Yue Lu |
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