- ￥1350 - 3000
- Proteintech
- 19157-1-AP
- 2025年10月13日
- WB, IHC, IP, ELISA
- Rabbit
- human, mouse, rat
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- 详细信息
- 询价记录
- 文献和实验
- 技术资料
- 免疫原：
Recombinant human TEK protein
- 亚型：
IgG
- 保存条件：
Store at -20°C. Stable for one year after shipment.
- 克隆性：
Polyclonal
- 标记物：
Unconjugated
- 适应物种：
human, mouse, rat
- 保质期：
详见产品包装
- 库存：
充足
- 宿主：
Rabbit
- 应用范围：
WB, IHC, IP, ELISA
- 靶点：
Tie-2/CD202b
- 抗体英文名：
Tie-2/CD202b Polyclonal antibody
- 抗体名：
Tie-2/CD202b Polyclonal antibody
- 规格：
50ul/100ul/150ul
| 规格： | 50ul | 产品价格： | ￥1350.0 |
|---|---|---|---|
| 规格： | 100ul | 产品价格： | ￥2200.0 |
| 规格： | 150ul | 产品价格： | ￥3000.0 |
经过测试的应用
| Positive WB detected in | mouse lung tissue, mouse liver tissue, Rat lung tissue |
| Positive IP detected in | mouse lung tissue |
| Positive IHC detected in | human placenta tissue, mouse kidney tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:1000-1:4000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
19157-1-AP targets Tie-2/CD202b in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, IP, ELISA |
| 文献引用应用 | WB, IHC, IF |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse, rat |
| 免疫原 |
CatNo: Ag13523 Product name: Recombinant human TEK protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 779-1124 aa of BC035514 Sequence: RRMAQAFQNVREEPAVQFNSGTLALNRKVKNNPDPTIYPVLDWNDIKFQDVIGEGNFGQVLKARIKKDGLRMDAAIKRMKEYASKDDHRDFAGELEVLCKLGHHPNIINLLGACEHRGYLYLAIEYAPHGNLLDFLRKSRVLETDPAFAIANSTASTLSSHHLLHFAADVARGMDYLSQKQFIHRDLAARNILVGENYVAKIADFGLSRGQEVYVKKTMGRLPVRWMAIESLNYSVYTTNSDVWSYGVLLWEIVSLGGTPYCGMTCAELYEKLPQGYRLEKPLNCDDEVYDLMRQCWREKPYERPSFAQILVSLNRMLEERKTYVNTTLYEKFTYAGIDCSAEEAA |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | TEK tyrosine kinase, endothelial |
| 别名 | TEK, Angiopoietin 1 receptor, Angiopoietin-1 receptor, CD202B, EC:2.7.10.1 |
| 计算分子量 | 1124 aa, 126 kDa |
| 观测分子量 | 140 kDa |
| GenBank蛋白编号 | BC035514 |
| 基因名称 | Tie2 |
| Gene ID (NCBI) | 7010 |
| RRID | AB_10644297 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q02763 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Tie2 (also known as TEK) is a tyrosine-protein kinase expressed almost exclusively on endothelial cells. It contains two immunoglobulin-like domains, three epidermal growth factor (EGF)--like domains and three fibronectin type III repeats. Tie2 acts as a cell-surface receptor for ANGPT1, ANGPT2, and ANGPT4 and regulates angiogenesis, endothelial cell survival, proliferation, migration, adhesion and cell spreading, reorganization of the actin cytoskeleton, but also maintenance of vascular quiescence. Mutations in the gene Tie2 are associated with inherited venous malformations of the skin and mucous membranes. Human Tie2 has a calculated molecular weight of 126 kDa. As a result of glycosylation, the apparent molecular mass of Tie2 is approximately 140-160 kDa.
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- 作者
- 内容
- 询问日期
文献和实验| Species | Application | Title |
|---|---|---|
| human | IHC | Ther Adv Med Oncol Development and external validation of a composite immune-clinical prognostic model associated with EGFR mutation in East-Asian patients with lung adenocarcinoma.Authors - Chengming Liu |
| human | IHC | Mol Cancer Ther JQ1 induces DNA damage and apoptosis, and inhibits tumor growth in a patient-derived xenograft model of cholangiocarcinoma.Authors - Patrick L Garcia |
| human | IF | Front Cell Dev Biol Inhibiting Heat Shock Protein 90 Protects Nucleus Pulposus-Derived Stem/Progenitor Cells From Compression-Induced Necroptosis and Apoptosis.Authors - Binwu Hu |
| human | WB | Stem Cell Res Ther Human endometrium-derived stem cell improves cardiac function after myocardial ischemic injury by enhancing angiogenesis and myocardial metabolism.Authors - Xuemei Fan |
| rat | WB | Stem Cell Res Ther MicroRNA-9 modified bone marrow-derived mesenchymal stem cells (BMSCs) repair severe acute pancreatitis (SAP) via inducing angiogenesis in rats.Authors - Daohai Qian |
| human | WB | J Mol Cell Cardiol BMP9 attenuates occurrence of venous malformation by maintaining endothelial quiescence and strengthening vessel walls via SMAD1/5/ID1/α-SMA pathway.Authors - Yongyun Li |
细胞激活；④通过转基因表达CD40L的成纤维细胞。也能刺激B细胞；⑤CD40或CD40L基因敲除的小鼠，抗体产生形成的缺陷类似人类的X性联高IgM血症，患者只产生了IgM，而无其他类别的抗体，也无生发中心，抗感染能力明显下降。 CD40与其配体CD401。的结合，导致CD40分子的寡聚化，使胞质中的TNF受体相关因子(TNF receptor-associ-atedfactor，TRAF)与CD40的胞内结构域结合。TRAF参与启动的酶催化级联反应，最终导致转录因子NFκB和AP-1等活化。
Southern Blotting: DNA Transfer
to 15 ml hyb solution for hybridization. 4. Incubate blot for at least 5 hours at 42℃...usually O/N. B. Washes and Immunochemiluminescent Detection 1. Pour off probe and save at -20℃in 50 ml conical tube. 2. Wash blot with Blot Wash #1: 2 x
R&D Systems:如何提高MSC的扩增且不损失其分化潜能
羊抗小鼠FABP-4多克隆抗体，PE非连接的小鼠抗人骨钙蛋白单克隆抗体，PE非连接的山羊抗人聚蛋白多糖多克隆抗体，PE连接和非连接的同型对照，NorthernLights-557连接的驴抗山羊和驴抗小鼠的二抗都采购于R&D Systems。PE连接的小鼠抗人CD34单克隆抗体源于BD Pharmingen（BD Biosciences，San Jose）。 流式细胞术。hMSCs的收集如上所述。细胞用2×冷FACS缓冲液冲洗（含有2%FBS和叠氮
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