- ￥1350 - 3000
- Proteintech
- 17625-1-AP
- 2025年10月13日
- IHC, ELISA
- Rabbit
- human, mouse
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- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
Recombinant human IL-21 protein
- 亚型：
IgG
- 保存条件：
Store at -20°C. Stable for one year after shipment.
- 克隆性：
Polyclonal
- 标记物：
Unconjugated
- 适应物种：
human, mouse
- 保质期：
详见产品包装
- 库存：
充足
- 宿主：
Rabbit
- 应用范围：
IHC, ELISA
- 靶点：
IL-21
- 抗体英文名：
IL-21 Polyclonal antibody
- 抗体名：
IL-21 Polyclonal antibody
- 规格：
50ul/100ul/150ul
| 规格： | 50ul | 产品价格： | ￥1350.0 |
|---|---|---|---|
| 规格： | 100ul | 产品价格： | ￥2200.0 |
| 规格： | 150ul | 产品价格： | ￥3000.0 |
经过测试的应用
| Positive IHC detected in | mouse skin tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
17625-1-AP targets IL-21 in IHC, IF, ELISA applications and shows reactivity with human, mouse samples.
| 经测试应用 | IHC, ELISA |
| 文献引用应用 | IHC, IF |
| 经测试反应性 | human, mouse |
| 文献引用反应性 | human, mouse |
| 免疫原 |
CatNo: Ag11838 Product name: Recombinant human IL-21 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 31-162 aa of BC066258 Sequence: GQDRHMIRMRQLIDIVDQLKNYVNDLVPEFLPAPEDVETNCEWSAFSCFQKAQLKSANTGNNERIINVSIKKLKRKPPSTNAGRRQKHRLTCPSCDSYEKKPPKEFLERFKSLLQKMIHQHLSSRTHGSEDS |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | interleukin 21 |
| 别名 | IL 21, IL21, IL-21, interleukin 21, Za11 |
| 计算分子量 | 162 aa, 19 kDa |
| GenBank蛋白编号 | BC066258 |
| 基因名称 | IL-21 |
| Gene ID (NCBI) | 59067 |
| RRID | AB_2878422 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q9HBE4 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Interleukin 21 (IL-21) is a cytokine produced predominantly by cluster of differentiation 4 (CD4+) T-cells and natural killer T-cells. IL-21 is preliminarily expressed in activated cluster of differentiation 4 (CD4+) T-cells and natural killer (NK) T-cells. IL-21 receptor (IL-21R) is a class I cytokine receptor, which requires dimerization with the indispensable common gamma chain (γc) subunit to bind IL-21. IL-21 plays a role in both the innate and adaptive immune responses by inducing the differentiation, proliferation and activity of multiple target cells including macrophages, natural killer cells, B cells and cytotoxic T cells. IL-21 mediates apoptosis in B-CLL cells through up-regulation of the BIM (BH3 family member) and enhances both direct and antibody-dependent cellular cytotoxicity in these cells.
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文献和实验| Species | Application | Title |
|---|---|---|
| mouse | FC | Mol Cell Neurosci Statin-modified dendritic cells regulate humoral immunity in experimental autoimmune myasthenia gravis.Authors - Heng Li |
| mouse | IHC | Biosci Biotechnol Biochem Interleukin-21 knockout reduces bone loss in ovariectomized mice by inhibiting osteoclastogenesisAuthors - Junlong Hou |
| human | IF | Int J Mol Sci Single-Cell RNA-Sequencing Reveals Peripheral T Helper Cells Promoting the Development of IgG4-Related Disease by Enhancing B Cell Activation and DifferentiationAuthors - Zongfei Ji |
| human | IHC | FASEB J IL-21 promoting angiogenesis contributes to the development of psoriasisAuthors - Dan Han |
| mouse | Immunol Res Dual neutralization of TGF-β and IL-21 regulates Th17/Treg balance by suppressing inflammatory signalling in the splenic lymphocytes of Staphylococcus aureus infection-induced septic arthritic miceAuthors - Rochana Pramanik | |
| mouse | IF | Int Immunopharmacol The causative effect of CXCR7 on experimental autoimmune prostatitis injury and fibrosisAuthors - Yi Zhang |
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实验步骤 1. 蛋白螯合 根据UV吸光值测量半抗原/蛋白的摩尔比率评价半抗原螯合蛋白(BSA，OVA)。半抗原/蛋白的摩尔比率基本一致，磺胺类药物/蛋白质的摩尔比率范围为20至40；而免疫原和螯合物的比率为3至9；对HRP螯合半抗原/HRPd摩尔比率范围为2至3。 2. 免疫 免疫原(0.20mg/0.5mLPBS)溶解于0.5mL弗氏完全佐剂中，肌肉注射于雌新西兰白兔(I和II)。间隔21天注射相同剂量
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