- ￥1350 - 3000
- Proteintech
- 15171-1-AP
- 2025年10月13日
- WB, IHC, IF/ICC, ELISA
- Rabbit
- human, mouse, rat
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- 文献和实验
- 技术资料
- 免疫原：
Recombinant human GPI protein
- 亚型：
IgG
- 保存条件：
Store at -20°C. Stable for one year after shipment.
- 克隆性：
Polyclonal
- 标记物：
Unconjugated
- 适应物种：
human, mouse, rat
- 保质期：
详见产品包装
- 库存：
充足
- 宿主：
Rabbit
- 应用范围：
WB, IHC, IF/ICC, ELISA
- 靶点：
GPI
- 抗体英文名：
GPI Polyclonal antibody
- 抗体名：
GPI Polyclonal antibody
- 规格：
50ul/100ul/150ul
| 规格： | 50ul | 产品价格： | ￥1350.0 |
|---|---|---|---|
| 规格： | 100ul | 产品价格： | ￥2200.0 |
| 规格： | 150ul | 产品价格： | ￥3000.0 |
经过测试的应用
| Positive WB detected in | HeLa cells, PC-3 cells, PC3 cells, U251 cells, U87-MG cells |
| Positive IHC detected in | human lung cancer tissue, human normal colon Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | PC-3 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunohistochemistry (IHC) | IHC : 1:100-1:400 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
15171-1-AP targets GPI in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, IF/ICC, ELISA |
| 文献引用应用 | WB, IHC, IF |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse, rat, pig |
| 免疫原 |
CatNo: Ag7423 Product name: Recombinant human GPI protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-272 aa of BC004982 Sequence: MAALTRDPQFQKLQQWYREHRSELNLRRLFDANKDRFNHFSLTLNTNHGHILVDYSKNLVTEDVMRMLVDLAKSRGVEAARERMFNGEKINYTEGRAVLHVALRNRSNTPILVDGKDVMPEVNKVLDKMKSFCQRVRSGDWKGYTGKTITDVINIGIGGSDLGPLMVTEALKPYSSGGPRVWYVSNIDGTHIAKTLAQLNPESSLFIIASKTFTTQETITNAETAKEWFLQAAKDPSAVAKHFVALSTNTTKVKEFGIDPQNMFEFWDWVGG |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | glucose phosphate isomerase |
| 别名 | NLK, Neuroleukin, GNPI, Glucose-6-phosphate isomerase, EC:5.3.1.9 |
| 计算分子量 | 63 kDa |
| 观测分子量 | 55-64 kDa |
| GenBank蛋白编号 | BC004982 |
| 基因名称 | GPI |
| Gene ID (NCBI) | 2821 |
| RRID | AB_2263537 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | P06744 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
GPI(Glucose-6-phosphate isomerase), which is also named as autocrine motility factor (AMF), phosphoglucose isomerase(PGI), Neuroleukinis(NLK), phosphohexose isomerase(PHI) or sperm antigen 36(SA-36), is a housekeeping cytosolic enzyme that plays a key role in both glycolysis and gluconeogenesis pathways. It is also a multifunctional protein that displays cytokine properties, eliciting mitogenic, motogenic, and differentiation activities, and has been implicated in tumor progression and metastasis (PMID:12783864, 19603112). This protein can exsit as a homodimer in the catalytically active form and a monomer in the secreted form (PMID:11371164). It has 2 isoforms produced by alternative splicing with the calculated molecular mass of 63-64kDa, and an apparent molecular mass of 55 and 64 kDa under non-reducing and reducing conditions, respectively(PMID: 19603112, 11004567).
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文献和实验| Species | Application | Title |
|---|---|---|
| human | WB | Cell Res Mannose antagonizes GSDME-mediated pyroptosis through AMPK activated by metabolite GlcNAc-6PAuthors - Yuan-Li Ai |
| mouse | IF | Nat Neurosci A glycolytic shift in Schwann cells supports injured axons.Authors - Elisabetta Babetto |
| human | WB | Cell Res cMyc-mediated activation of serine biosynthesis pathway is critical for cancer progression under nutrient deprivation conditions.Authors - Linchong Sun |
| mouse | WB | EMBO J Fatty acid synthesis is critical for stem cell pluripotency via promoting mitochondrial fission.Authors - Lihua Wang |
| human | WB | EMBO Rep CUE domain-containing protein 2 promotes the Warburg effect and tumorigenesis.Authors - Xiuying Zhong |
| human | WB | EMBO Rep OMA1 reprograms metabolism under hypoxia to promote colorectal cancer development.Authors - Zhida Wu |
b～9 调控MAC的组装；保护精子活性 膜蛋白分子： MCP（CD46） 40～70（4个SCR，GPI锚） 除红细胞外的血细胞、上皮细胞、内皮细胞 C3b,C3b 作为I因子裂解C3b和C4b的辅助因子 DAF（CD55） 70（4个SCR，GPI锚） 绝大多数血细胞、内皮细胞、粘膜上皮细胞 C 4b2a,C 3bBb 抑制C3转酶形成 促进CP和AP中的C3转化酶衰变 HRF（C8bp） 65（GPI锚） 红细胞、PMN、单核细胞、淋巴细胞、血小板 C8、C9 抑制C9与C8
9.30 兰州大学 Nature 子刊发现共用转录因子调控新机制
Control of Atf4 ⑤ Cell 子刊：LLG2 / 3 作为复合体的共受体参与调控花粉管破裂花粉管的细胞完整性对精细胞的顺利传送至关重要，即花粉管既要在极性生长过程中保持一定的细胞完整性，防止精细胞提前释放，又要在胚珠中及时破裂，为双受精进一步进行准备。北京大学生科院瞿礼嘉课题组在系统地分析了拟南芥中的 GPI-AP 基因之后，发现了两个与 LRE/LLG1 相似、其相互之间序列又非常相似的 GPI-AP 基因 LLG2 和 LLG3，这两个基因在花粉中大量表达。随后他们采用 CRISPR
脂筏与信号途径 免疫细胞信号转导的通用简要流程中，受体跨膜分子附近的细胞膜形成一种特化性结构称为脂筏(1ipidraft)。这是一种能够抗去污剂作用，富含糖脂的微结构域(glycolipid -enrlchedmicrodomaln，GEM)，由鞘脂(spingolipid)、胆固醇和糖基磷脂酰肌醇(GPI)锚定蛋白等组成，质地较硬，能够像船筏一样漂浮在周围流动性较强的膜脂双层结构中。脂筏结构可不断变化，或碎成小片，或连接成大片，并与周围的细胞膜进行成分交换。免疫细胞静止时脂筏
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