- ￥1350 - 3000
- Proteintech
- 14858-1-AP
- 2025年10月13日
- WB, IHC, FC (Intra), ELISA
- Rabbit
- human, mouse, rat
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- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
Recombinant human SYK protein
- 亚型：
IgG
- 保存条件：
Store at -20°C. Stable for one year after shipment.
- 克隆性：
Polyclonal
- 标记物：
Unconjugated
- 适应物种：
human, mouse, rat
- 保质期：
详见产品包装
- 库存：
充足
- 宿主：
Rabbit
- 应用范围：
WB, IHC, FC (Intra), ELISA
- 靶点：
SYK
- 抗体英文名：
SYK Polyclonal antibody
- 抗体名：
SYK Polyclonal antibody
- 规格：
50ul/100ul/150ul
| 规格： | 50ul | 产品价格： | ￥1350.0 |
|---|---|---|---|
| 规格： | 100ul | 产品价格： | ￥2200.0 |
| 规格： | 150ul | 产品价格： | ￥3000.0 |
经过测试的应用
| Positive WB detected in | Daudi cells, Raji cells, Ramos cells |
| Positive IHC detected in | human liver cancer tissue, human appendicitis tissue, human spleen tissue, human kidney tissue, human ovary tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive FC (Intra) detected in | Ramos cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:3000 |
| Immunohistochemistry (IHC) | IHC : 1:400-1:1600 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
14858-1-AP targets SYK in WB, IHC, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, FC (Intra), ELISA |
| 文献引用应用 | WB, IHC |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse |
| 免疫原 |
CatNo: Ag6657 Product name: Recombinant human SYK protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 368-635 aa of BC001645 Sequence: KLLTLEDKELGSGNFGTVKKGYYQMKKVVKTVAVKILKNEANDPALKDELLAEANVMQQLDNPYIVRMIGICEAESWMLVMEMAELGPLNKYLQQNRHVKDKNIIELVHQVSMGMKYLEESNFVHRDLAARNVLLVTQHYAKISDFGLSKALRADENYYKAQTHGKWPVKWYAPECINYYKFSSKSDVWSFGVLMWEAFSYGQKPYRGMKGSEVTAMLEKGERMGCPAGCPREMYDLMNLCWTYDVENRPGFAAVELRLRNYYYDVVN |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | spleen tyrosine kinase |
| 别名 | Tyrosine-protein kinase SYK, Tyrosine protein kinase SYK, spleen tyrosine kinase, p72-Syk, IMD82 |
| 计算分子量 | 72 kDa |
| 观测分子量 | 66-72 kDa |
| GenBank蛋白编号 | BC001645 |
| 基因名称 | SYK |
| Gene ID (NCBI) | 6850 |
| ENSEMBL Gene ID | ENSG00000165025 |
| RRID | AB_2878086 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | P43405 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
SYK belongs to the protein kinase superfamily, Tyr protein kinase family and SYK/ZAP-70 subfamily. It is positive effector of BCR-stimulated responses. SYK couples the B-cell antigen receptor (BCR) to the mobilization of calcium ion either through a phosphoinositide 3-kinase-dependent pathway, when not phosphorylated on tyrosines of the linker region, or through a phospholipase C-gamma-dependent pathway It phosphorylates USP25 and regulates its intracellular levels. SYK also promotes liver fibrosis by activation of hepatic stellate cells and acts as a biomarker for human hepatocellular carcinoma. (PMID: 29537660) SYK can be detected 66-72 kDa in human and mouse (PMID: 37661351).
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文献和实验| Species | Application | Title |
|---|---|---|
| mouse | WB | Food Funct Synergistic effects of L-theanine and epigallocatechin gallate in alleviating ovalbumin allergy by regulating intestinal immunity through inhibition of mast cell degranulationAuthors - Wei Xu |
| mouse | WB | Int Immunopharmacol SAP130 released by ferroptosis tubular epithelial cells promotes macrophage polarization via Mincle signaling in sepsis acute kidney injuryAuthors - Jing Zhang |
| mouse | IHC | J Ethnopharmacol Yiqi Kaimi prescription regulates protein phosphorylation to promote intestinal motility in slow transit constipationAuthors - Yi-Bo Yao |
| mouse | WB | Brain Res Inhibition of TREM-1 alleviates neuroinflammation by modulating microglial polarization via SYK/p38MAPK signaling pathway after traumatic brain injuryAuthors - Tianqi Zhao |
| mouse | WB | Int J Biol Macromol A nanobody-enzyme fusion protein targeting PD-L1 and sialic acid exerts anti-tumor effects by C-type lectin pathway-mediated tumor associated macrophages repolarizingAuthors - Yongliang Tong |
| human | IHC | Front Genet SYK Is Associated With Malignant Phenotype and Immune Checkpoints in Diffuse Glioma.Authors - Quanwei Zhou |
膜结合的衔接蛋白是连接淋巴细胞激活信号胞内传递的重要成分，T细胞中为I-AT．B细胞中称为B细胞连接蛋白(Bcelllinkerprotein，BLNK)。前面提到，游离于胞质中的Syk-PTK被招募并激活后，其作用的靶分子之一，即是含SH2结构域的BLNK，并通过BI。NK构筑的“平台”，吸引并进一步激活多种信号分子包括蛋白激酶和其他衔接蛋白，Tec-PTK家族的Bruton酪氨酸激酶(Btk)、磷脂酶C的γ链和衔接蛋白SLP-65。Btk接受来自Syk的磷酸根而激活并使PlC-γ活化
完全活化成为效应T细胞。在正常生理情况下，共刺激分子与共抑制分子之间的平衡，使T细胞的免疫效应保持适当的深度、广度，从而最大程度减少对于周围正常组织的损伤，维持对自身组织的耐受、避免自身免疫反应。然而，肿瘤细胞可异常上调共抑制分子PD-1、PD-L1的含量。PD-L1与PD-1结合后，PD-1胞质区ITSM结构域中的酪氨酸发生磷酸化，募集SHP-2磷酸酶，使下游的Syk和磷脂酰肌醇3激酶(PI3K)发生去磷酸化，从而传递抑制性信号抑制淋巴细胞的功能及细胞因子的释放，打破共刺激分子与共抑制分子之间
因此，综合来说，EarthOx的GST标签抗体 （货号#E022040）是一款同时具有高特异性、高灵敏度、多应用类型以及性价比的GST标签抗体。同时，我们也提供其他品牌的GST标签抗体，如Proteintech的GST多克隆抗体 ――GST PolyClonal Antibody（货号#10000-0-AP）以及Abcam、Santa等多种GST标签抗体产品。更多GST标签抗体以及其他如His、HA、Myc、GFP、Flag等其他标签抗体，请电话垂询。美国EarthOx品牌是一家提供高性价比免疫学和细胞
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