- ￥1350 - 3000
- Proteintech
- 14503-1-AP
- 2025年10月13日
- WB, IF/ICC, FC (Intra), IP, ELISA
- Rabbit
- human, mouse, rat
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- 文献和实验
- 技术资料
- 免疫原：
Recombinant human 14-3-3 protein
- 亚型：
IgG
- 保存条件：
Store at -20°C. Stable for one year after shipment.
- 克隆性：
Polyclonal
- 标记物：
Unconjugated
- 适应物种：
human, mouse, rat
- 保质期：
详见产品包装
- 库存：
充足
- 宿主：
Rabbit
- 应用范围：
WB, IF/ICC, FC (Intra), IP, ELISA
- 靶点：
14-3-3
- 抗体英文名：
14-3-3 Polyclonal antibody
- 抗体名：
14-3-3 Polyclonal antibody
- 规格：
50ul/100ul/150ul
| 规格： | 50ul | 产品价格： | ￥1350.0 |
|---|---|---|---|
| 规格： | 100ul | 产品价格： | ￥2200.0 |
| 规格： | 150ul | 产品价格： | ￥3000.0 |
经过测试的应用
| Positive WB detected in | HeLa cells, mouse liver tissue, mouse brain tissue, Jurkat cells, rat liver tissue, mouse heart tissue, NIH/3T3 cells, C6 cells |
| Positive IP detected in | mouse lung tissue |
| Positive IF/ICC detected in | HepG2 cells |
| Positive FC (Intra) detected in | HeLa cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
14503-1-AP targets 14-3-3 in WB, IHC, IF/ICC, FC (Intra), IP, CoIP, RIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IF/ICC, FC (Intra), IP, ELISA |
| 文献引用应用 | WB, IHC, IF, IP, CoIP, RIP |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse, rat |
| 免疫原 |
CatNo: Ag5959 Product name: Recombinant human 14-3-3 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-245 aa of BC056867 Sequence: MEKTELIQKAKLAEQAERYDDMATCMKAVTEQGAELSNEERNLLSVAYKNVVGGRRSAWRVISSIEQKTDTSDKKLQLIKDYREKVESELRSICTTVLELLDKYLIANATNPESKVFYLKMKGDYFRYLAEVACGDDRKQTIDNSQGAYQEAFDISKKEMQPTHPIRLGLALNFSVFYYEILNNPELACTLAKTAFDEAIAELDTLNEDSYKDSTLIMQLLRDNLTLWTSDSAGEECDAAEGAEN |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, theta polypeptide |
| 别名 | YWHAQ, HS1, 1C5, 14-3-3σ, 14-3-3 zeta |
| 计算分子量 | 28 kDa |
| 观测分子量 | 31 kDa |
| GenBank蛋白编号 | BC056867 |
| 基因名称 | 14-3-3 theta |
| Gene ID (NCBI) | 10971 |
| RRID | AB_2218096 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | P27348 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
14-3-3 proteins are the first phosphoserine/phosphothreonine-binding proteins to be discovered. 14-3-3 family members interact with a wide spectrum of proteins and possess diverse functions. Mammals express seven distinct 14-3-3 isoforms (gamma, epsilon, beta, zeta, sigma, theta, tau) that form multiple homo- and hetero- dimmers. 14-3-3 proteins display the highest expression levels in the brain, and have been implicated in several neurodegenerative diseases, including Alzheimer's disease and amyotrophic lateral sclerosis.
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文献和实验| Species | Application | Title |
|---|---|---|
| human | WB | Neuron Targeting 14-3-3θ-mediated TDP-43 pathology in amyotrophic lateral sclerosis and frontotemporal dementia miceAuthors - Yazi D Ke |
Int J Biol Macromol Biofilm formation initiating rotifer-specific biopolymer and its predicted componentsAuthors - Zsolt Datki | ||
| human | WB | Elife Ser/Thr kinase Trc controls neurite outgrowth in Drosophila by modulating microtubule-microtubule sliding.Authors - Rosalind Norkett
|
| human | CoIP | Int J Mol Sci APEX2-Mediated Proximity Labeling Resolves the DDIT4-Interacting Proteome.Authors - Marianna Naki |
| human,mouse | WB,IP | Aging (Albany NY) The interaction protein of SORBS2 in myocardial tissue to find out the pathogenic mechanism of LVNC disease.Authors - Chunyan Li |
| human | WB | J Inflamm Res PADI6 Regulates Trophoblast Cell Migration-Invasion Through the Hippo/YAP1 Pathway in Hydatidiform Moles.Authors - Bo Huang |
AP Assay and FGF Binding Mike Naski COS-7 cells were transfected by a standard DEAE-dextran method with 4 mg of plasmid/10 6 cells. Conditioned medium was harvested 3, 6 and 9 days after transfection and used for AP enzyme
Stem Cell Rep：高绍荣团队揭示 Mettl14 以不依赖于 m⁶A 的方式驱动衰老相关分泌表型促进体细胞重编程
的方式调控 SASP 基因表达水平 6。因此，研究者推测，在重编程晚期，Mettl14 上调的这些基因为 SASP 基因。进一步研究发现，在重编程晚期，这些 SASP 基因的表达水平显著升高，但是，在建系的 iPSC 中几乎不表达。 为了进一步研究 SASP 与重编程的关系，研究者进行了细胞上清共培养实验及 SASP 因子共培养实验 (图 2)。结果表明，重编程过程的细胞上清或 SASP 因子（如 IL6）可以促进重编程后期的细胞转变为多能干细胞。同时，对细胞进行碱性磷酸酶 AP 染色和 β
流速为14～16滴/min。分部收集器收集，紫外监测，或人工收集，以紫外分光光度计分别测定每管280nm OD值，描绘洗脱液峰。 6. 浓缩：将洗脱液的280nm OD上峰段与下峰段各管分别合并，装入透析袋，以PEG浓缩至所需体积。 三、Tris-PO4 离子交换层析法 该法在上述方法的基础上稍加改良，即通过梯度洗脱，可用于血清中IgG和IgM的提取。 材料和试剂 1. DE32或DE52纤维素。 2. 待纯化标本：杂交瘤腹水或培养上清，免疫血清或饱和硫酸铵粗提品。
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