- ￥1350 - 3000
- Proteintech
- 14427-1-AP
- 2025年10月13日
- WB, IHC, IF/ICC, IF-P, FC (Intra), ELISA
- Rabbit
- human, mouse
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- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
Recombinant human APOA1 protein
- 亚型：
IgG
- 保存条件：
Store at -20°C. Stable for one year after shipment.
- 克隆性：
Polyclonal
- 标记物：
Unconjugated
- 适应物种：
human, mouse
- 保质期：
详见产品包装
- 库存：
充足
- 宿主：
Rabbit
- 应用范围：
WB, IHC, IF/ICC, IF-P, FC (Intra), ELISA
- 靶点：
Apolipoprotein AI
- 抗体英文名：
Apolipoprotein AI Polyclonal antibody
- 抗体名：
Apolipoprotein AI Polyclonal antibody
- 规格：
50ul/100ul/150ul
| 规格： | 50ul | 产品价格： | ￥1350.0 |
|---|---|---|---|
| 规格： | 100ul | 产品价格： | ￥2200.0 |
| 规格： | 150ul | 产品价格： | ￥3000.0 |
经过测试的应用
| Positive WB detected in | human blood tissue, human brain tissue, human plasma, human ileum tissue |
| Positive IHC detected in | human liver cancer tissue, human liver tissue, human lung tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF-P detected in | mouse liver tissue |
| Positive IF/ICC detected in | HeLa cells |
| Positive FC (Intra) detected in | HepG2 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
14427-1-AP targets Apolipoprotein AI in WB, IHC, IF/ICC, IF-P, FC (Intra), IP, ELISA applications and shows reactivity with human, mouse samples.
| 经测试应用 | WB, IHC, IF/ICC, IF-P, FC (Intra), ELISA |
| 文献引用应用 | WB, IHC, IF, IP |
| 经测试反应性 | human, mouse |
| 文献引用反应性 | human, mouse |
| 免疫原 |
CatNo: Ag5793 Product name: Recombinant human APOA1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 19-267 aa of BC005380 Sequence: RHFWQQDEPPQSPWDRVKDLATVYVDVLKDSGRDYVSQFEGSALGKQLNLKLLDNWDSVTSTFSKLREQLGPVTQEFWDNLEKETEGLRQEMSKDLEEVKAKVQPYLDDFQKKWQEEMELYRQKVEPLRAELQEGARQKLHELQEKLSPLGEEMRDRARAHVDALRTHLAPYSDELRQRLAARLEALKENGGARLAEYHAKATEHLSTLSEKAKPALEDLRQGLLPVLESFKVSFLSALEEYTKKLNTQ |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | apolipoprotein A-I |
| 别名 | APOA1, apolipoprotein A I, ApoA-I, Apo-AI, ApoAI |
| 计算分子量 | 31 kDa |
| 观测分子量 | 26-30 kDa |
| GenBank蛋白编号 | BC005380 |
| 基因名称 | APOA1 |
| Gene ID (NCBI) | 335 |
| RRID | AB_2056524 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | P02647 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
ApoA1 is a major protein component of high density lipoproteins (HDL) which is associated with reversed cholesterol transport, lipid/cholesterol binding, lecithin/cholesterol acyltransferase (LCAT) activation and specific receptors binding. It is synthesized in the liver and small intestine. Defects of ApoA1 cause low HDL level and systemic non-neuropathic amyloidosis. Serum concentration of ApoA1 is inversely related to the risk of developing atherosclerosis. This antibody was generated against the C-terminal region of human ApoA1.
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文献和实验| Species | Application | Title |
|---|---|---|
| IP | Science Enterically derived high-density lipoprotein restrains liver injury through the portal vein.Authors - Yong-Hyun Han | |
| mouse | WB,IP | Neuron Astrocytic ApoE reprograms neuronal cholesterol metabolism and histone-acetylation-mediated memory.Authors - Xiaohui Li |
| mouse | WB | J Control Release Lipid-mediated protein corona regulation with increased apolipoprotein A-I recruitment for glioma targetingAuthors - Yiwei Zhang |
| human | IHC | Br J Cancer A new panel of pancreatic cancer biomarkers discovered using a mass spectrometry-based pipeline.Authors - Xiaohui Liu |
| human | WB | Theranostics Exosome-mediated delivery of inflammation-responsive Il-10 mRNA for controlled atherosclerosis treatmentAuthors - Te Bu |
Br J Pharmacol A novel apoA-I mimetic peptide suppresses atherosclerosis by promoting physiological HDL function in apoE-/- mice.Authors - Sanhu Gou |
Formation of Apolipoprotein AI-AII Heterodimers by Oxidation of High-Density Lipoprotein
Uptake of oxidized low-density lipoprotein (LDL) by scavenger receptors allows cholesterol to be accumulated in an unregulated fashion by cells in the artery wall, an event considered pivotal to the formation of atherosclerotic plaques
Immunity：北大蒋争凡课题组发现 STING 后高尔基体囊泡转运的重要性及机制
4KB 与 ARMH3 互作的关键区域（6 个氨基酸残基）都会显著削弱 cGAS-STING 通路的激活。这些结果表明 ARMH3 招募 PI4KB 对于 STING 的激活十分关键。 更重要的是，细胞内敲除 ARMH3 或者原位删除 PI4KB 结合 ARMH3 所需的 6 个氨基酸，STING 在上游通路活化后几乎呈现完全的内质网定位，暗示如果 2'3'-cGAMP 结合后被转运到高尔基体的 STING 无法被继续转运到后高尔基体囊泡，将会被细胞内 COPI/AP1 介导的从高尔基体向内质网的逆向转运运回
and its risk actors. Atherosclerosis,1984,36:6774 3．Maviejko J,Holmes R,Kottke BA,et al.Apolipoprotein AI as a marker of angiographically assessed artery,N Engl J Med,1983,309:385-389 4．Vander GL,Barbank J,Sasse EA,Correlateion of the extent of a new
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