相关产品推荐更多 >
万千商家帮你免费找货
0 人在求购买到急需产品
- 详细信息
- 文献和实验
- 技术资料
- 免疫原:
Recombinant human ARNT,HIF1B protein
- 亚型:
IgG
- 保存条件:
Store at -20°C. Stable for one year after shipment.
- 克隆性:
Polyclonal
- 标记物:
Unconjugated
- 适应物种:
human, mouse
- 保质期:
详见产品包装
- 库存:
充足
- 宿主:
Rabbit
- 应用范围:
WB, IHC, IF/ICC, IP, ELISA
- 靶点:
ARNT/HIF-1 beta
- 抗体英文名:
ARNT/HIF-1 beta Polyclonal antibody
- 抗体名:
ARNT/HIF-1 beta Polyclonal antibody
- 规格:
50ul/100ul/150ul
| 规格: | 50ul | 产品价格: | ¥1350.0 |
|---|---|---|---|
| 规格: | 100ul | 产品价格: | ¥2200.0 |
| 规格: | 150ul | 产品价格: | ¥3000.0 |
经过测试的应用
| Positive WB detected in | HEK-293 cells, HeLa cells, HepG2 cells |
| Positive IP detected in | HepG2 cells |
| Positive IHC detected in | mouse embryo tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | A431 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
14105-1-AP targets ARNT/HIF-1 beta in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse samples.
| 经测试应用 | WB, IHC, IF/ICC, IP, ELISA |
| 文献引用应用 | WB, IHC, IF, IP |
| 经测试反应性 | human, mouse |
| 文献引用反应性 | human, mouse, rat, pig |
| 免疫原 |
CatNo: Ag5238 Product name: Recombinant human ARNT,HIF1B protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-348 aa of BC060838 Sequence: MAATTANPEMTSDVPSLGPAIASGNSGPGIQGGGAIVQRAIKRRPGLDFDDDGEGNSKFLRCDDDQMSNDKERFARSDDEQSSADKERLARENHSEIERRRRNKMTAYITELSDMVPTCSALARKPDKLTILRMAVSHMKSLRGTGNTSTDGSYKPSFLTDQELKHLILEAADGFLFIVSCETGRVVYVSDSVTPVLNQQQSEWFGSTLYDQVHPDDVDKLREQLSTSENALTGRILDLKTGTVKKEGQQSSMRMCMGSRRSFICRMRCGSSSVDPVSVNRLSFVRNRCRNGLGSVKDGEPHFVVVHCTGYIKAWPPAGVSLPDDDPEAGQGSKFCLVAIGRLQVTSS |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | aryl hydrocarbon receptor nuclear translocator |
| 别名 | ARNT, HIF-1 beta, HIF1B, bHLHe2, Class E basic helix-loop-helix protein 2 |
| 计算分子量 | 416 aa, 46 kDa, 87 kDa |
| 观测分子量 | 87 kDa |
| GenBank蛋白编号 | BC060838 |
| 基因名称 | ARNT/HIF-1 beta |
| Gene ID (NCBI) | 405 |
| RRID | AB_10639523 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | P27540 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
HIF1B(HIF-1 beta), also named as ARNT, is a 789 amino acid protein, which forms a heterodimer with AHR, AHRR, HIF1A and EPAS1/HIF2A as well as with other bHLH proteins. HIF1B is Required for activity of the Ah (dioxin) receptor. This protein is required for the ligand-binding subunit to translocate from the cytosol to the nucleus after ligand binding. The heterodimer with HIF1A or EPAS1/HIF2A functions as a transcriptional regulator of the adaptive response to hypoxia
风险提示:丁香通仅作为第三方平台,为商家信息发布提供平台空间。用户咨询产品时请注意保护个人信息及财产安全,合理判断,谨慎选购商品,商家和用户对交易行为负责。对于医疗器械类产品,请先查证核实企业经营资质和医疗器械产品注册证情况。
文献和实验| Species | Application | Title |
|---|---|---|
| human | WB | Hepatology PROX1 promotes hepatocellular carcinoma metastasis by way of up-regulating hypoxia-inducible factor 1α expression and protein stability.Authors - Liu Yanfeng Y |
| human | WB,IF | BMC Med NUPR1 contributes to radiation resistance by maintaining ROS homeostasis via AhR/CYP signal axis in hepatocellular carcinomaAuthors - Yizhi Zhan |
| human | WB | Free Radic Biol Med Sestrin2 inhibits hypoxia-inducible factor-1α accumulation via AMPK-mediated prolyl hydroxylase regulation.Authors - Kyuhwa Seo |
| mouse | WB | Cell Immunol Fine particulate matter (PM2.5) induces inhibitory memory alveolar macrophages through the AhR/IL-33 pathwayAuthors - Yanan Liu |
| mouse | WB | Front Microbiol Fine Particulate Matter (PM2.5) Promotes CD146 Expression in Alveolar Epithelial Cells and Cryptococcus neoformans Pulmonary Infection.Authors - Zhixiao Sun |
| mouse | WB | J Biomed Nanotechnol Fine Particulate Matter (PM2.5) Promoted the Invasion of Lung Cancer Cells via an ARNT2/PP2A/STAT3/MMP2 Pathway.Authors - Zhongqi Chen |
真爱满行囊 我最近在体外细胞做的关于gsk3-beta功能问题,发现药物处理以后,gsk-3-beta的总表达量下调,如果是这样的话 1、我是否能够得出gsk-3-beta的活性下调的结论? 2、是否还有必要做非活性形式的表达量?我的理解是,基础状态下,gsk-3beta维持的是低活性状态,如果总量都下调了,那应该能够得出活性降低的结论了吧? 3、另外,在这种情况下我是否有必要去做gsk-3-beta的216位点的活性形式的表达
2. 抗原与填料的连接 这一步是整个纯化过程中最关键的一步,如果在这一步上有什么失误,建议你从step one重新开始吧。 首先要注意一点:常见的连接方法都基于抗原上的游离氨基或者巯基与活化后的beads的连接,因此体系中不应该有其它含有氨基或巯基的化合物,包括尿素、硫脲、盐酸胍、Tris、铵根离子、Beta-巯基乙醇等,如果有这些物质在体系中,应该首先设法除去。 溴化氰活化的琼脂糖与氨基化合物反应的机理如下: 环氧氯丙烷活化的琼脂糖与氨基化合物反应
文章:推测损坏神经元的 Aβ在大脑中进行着秘密活动——保护大脑免于病原体的侵袭。研究者发现 Aβ能够以「抗菌肽」的身份防御微生物感染;而且,对于一些微生物而言,它的抗菌效果超出青霉素 100 倍!因此,Aβ或许不需要完全清除。 人类越来越强大,面对最后一大块未被征服的科学领域——脑,也在想方设法的探索它的「私生活」。在探索的道路上,华美已经为您准备好了 APP 的重组蛋白、多克隆抗体和单克隆抗体。 淀粉样肽 beta A4 重组蛋白 货号:CSB-RP
技术资料暂无技术资料 索取技术资料










