- ￥1350 - 3000
- Proteintech
- 13884-1-AP
- 2026年05月21日
- WB, IHC, IF/ICC, ELISA
- Rabbit
- human, mouse, rat
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- 文献和实验
- 技术资料
- 免疫原：
Recombinant human SLC12A2 protein
- 亚型：
IgG
- 保存条件：
Store at -20°C. Stable for one year after shipment.
- 克隆性：
Polyclonal
- 标记物：
Unconjugated
- 适应物种：
human, mouse, rat
- 保质期：
详见产品包装
- 库存：
充足
- 宿主：
Rabbit
- 应用范围：
WB, IHC, IF/ICC, ELISA
- 靶点：
NKCC1/SLC12A2
- 抗体英文名：
NKCC1/SLC12A2 Polyclonal antibody
- 抗体名：
NKCC1/SLC12A2 Polyclonal antibody
- 规格：
50ul/100ul/150ul
| 规格： | 50ul | 产品价格： | ￥1350.0 |
|---|---|---|---|
| 规格： | 100ul | 产品价格： | ￥2200.0 |
| 规格： | 150ul | 产品价格： | ￥3000.0 |
经过测试的应用
| Positive WB detected in | mouse brain tissue, 37°C incubated mouse brain tissue |
| Positive IHC detected in | human colon cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | A549 cells |
For optimal WB detection with 13884-1-AP, we do not recommend boiling the sample after lysis.
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
13884-1-AP targets NKCC1/SLC12A2 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, IF/ICC, ELISA |
| 文献引用应用 | WB, IHC, IF, IP |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse, rat, pig |
| 免疫原 |
CatNo: Ag4991 Product name: Recombinant human SLC12A2 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 759-1150 aa of BC033003 Sequence: GSSTQALTYLNALQHSIRLSGVEDHVKNFRPQCLVMTGAPNSRPALLHLVHDFTKNVGLMICGHVHMGPRRQAMKEMSIDQAKYQRWLIKNKMKAFYAPVHADDLREGAQYLMQAAGLGRMKPNTLVLGFKKDWLQADMRDVDMYINLFHDAFDIQYGVVVIRLKEGLDISHLQGQEELLSSQEKSPGTKDVVVSVEYSKKSDLDTSKPLSEKPITHKVEEEDGKTATQPLLKKESKGPIVPLNVADQKLLEASTQFQKKQGKNTIDVWWLFDDGGLTLLIPYLLTTKKKWKDCKIRVFIGGKINRIDHDRRAMATLLSKFRIDFSDIMVLGDINTKPKKENIIAFEEIIEPYRLHEDDKEQDIADKMKEDEPWRITDNELELYKTKFYEPC |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | solute carrier family 12 (sodium/potassium/chloride transporters), member 2 |
| 别名 | NKCC1,SLC12A2, NKCC1, SLC12A2, Basolateral Na K Cl symporter, Basolateral Na-K-Cl symporter |
| 计算分子量 | 131 kDa |
| 观测分子量 | 150-160 kDa |
| GenBank蛋白编号 | BC033003 |
| 基因名称 | NKCC1/SLC12A2 |
| Gene ID (NCBI) | 6558 |
| RRID | AB_2188522 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | P55011 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
The Na-K-Cl cotransporter sodium-potassium-chloride cotransporter 1 (NKCC1), encoded by the SLC12A2 gene, is an intensively studied member of the CCC family. NKCC1 is a membrane protein and plays fundamental roles in regulating trans-epithelial ion movement, cell volume, chloride homeostasis and neuronal excitability. NKCC1 assembles into a dimer, with the first ten transmembrane (TM) helices harboring the transport core and TM11-TM12 helices lining the dimer interface(PMID: 32081947). The observed molecular weight of NKCC1 is about 140 kDa, glycosylated NKCC1 is detected a band at ∼170 kDa (PMID: 22723696).
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文献和实验| Species | Application | Title |
|---|---|---|
| mouse | WB,IHC | Science A causative link between inner ear defects and long-term striatal dysfunction.Authors - Michelle W Antoine |
| mouse | IHC | PLoS Biol Early uneven ear input induces long-lasting differences in left-right motor function.Authors - Michelle W Antoine |
| mouse | IF | Front Immunol Myeloid-derived growth factor promotes M2 macrophage polarization and attenuates Sjögren's syndrome via suppression of the CX3CL1/CX3CR1 axisAuthors - Zi Yang |
| mouse | IHC | PLoS Biol The NKCC1 ion transporter modulates microglial phenotype and inflammatory response to brain injury in a cell-autonomous manner.Authors - Krisztina Tóth |
| mouse | WB,IHC | J Neurosci The severity of vestibular dysfunction in deafness as a determinant of comorbid hyperactivity or anxiety.Authors - Michelle W Antoine |
| mouse | IF | Front Mol Neurosci Unilateral optogenetic kindling of hippocampus leads to more severe impairments of the inhibitory signaling in the contralateral hippocampusAuthors - Fabio Cesar Tescarollo |
因此，综合来说，EarthOx的GST标签抗体 （货号#E022040）是一款同时具有高特异性、高灵敏度、多应用类型以及性价比的GST标签抗体。同时，我们也提供其他品牌的GST标签抗体，如Proteintech的GST多克隆抗体 ――GST PolyClonal Antibody（货号#10000-0-AP）以及Abcam、Santa等多种GST标签抗体产品。更多GST标签抗体以及其他如His、HA、Myc、GFP、Flag等其他标签抗体，请电话垂询。美国EarthOx品牌是一家提供高性价比免疫学和细胞
Microarray Approaches for Analysis of Cell Cycle Regulatory Genes
Cell cycle phase Mammalian Yeast G0/G1 AP-1 family (FOS, JUN)
同时，Apaf―1可以激活原形的caspase―9，形成细胞色素C／Apaf―1／caspase―9复合体，即凋亡复合体(apoptosome)。该复合体水解并激活下游的caspase通路如caspase―3等。 凋亡复合体的形成是凋亡信号启动的关键步骤。目前发现激活的癌基因可以辅助凋亡复合体的形成，比如腺病毒EIA可以上调Apaf―1和caspase―9，E2F1可以上调Apaf―1的表达。Apaf―1的释放可促进凋亡复合体的形成，对凋亡的启动起促进作用。目前的研究认为，细胞
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