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- 详细信息
- 文献和实验
- 技术资料
- 免疫原:
Recombinant human TBX5 protein
- 亚型:
IgG
- 保存条件:
Store at -20°C. Stable for one year after shipment.
- 克隆性:
Polyclonal
- 标记物:
Unconjugated
- 适应物种:
human, mouse, rat
- 保质期:
详见产品包装
- 库存:
充足
- 宿主:
Rabbit
- 应用范围:
WB, IF/ICC, ELISA
- 靶点:
TBX4/5
- 抗体英文名:
TBX4/5 Polyclonal antibody
- 抗体名:
TBX4/5 Polyclonal antibody
- 规格:
50ul/100ul/150ul
| 规格: | 50ul | 产品价格: | ¥1350.0 |
|---|---|---|---|
| 规格: | 100ul | 产品价格: | ¥2200.0 |
| 规格: | 150ul | 产品价格: | ¥3000.0 |
经过测试的应用
| Positive WB detected in | HeLa cells, HepG2 cells, MCF-7 cells |
| Positive IF/ICC detected in | HepG2 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:10-1:100 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
13178-1-AP targets TBX4/5 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IF/ICC, ELISA |
| 文献引用应用 | WB, IF |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse |
| 免疫原 |
CatNo: Ag3845 Product name: Recombinant human TBX5 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-328 aa of BC027942 Sequence: MADADEGFGLAHTPLEPDAKDLPCDSKPESALGAPSKSPSSPQAAFTQQGMEGIKVFLHERELWLKFHEVGTEMIITKAGRRMFPSYKVKVTGLNPKTKYILLMDIVPADDHRYKFADNKWSVTGKAEPAMPGRLYVHPDSPATGAHWMRQLVSFQKLKLTNNHLDPFGHIILNSMHKYQPRLHIVKADENNGFGSKNTAFCTHVFPETAFIAVTSYQNHKITQLKIENNPFAKGFRGSDDMELHRMSRMQSKEYPVVPRSTVRQKVASNHSPFSSESRALSTSSNLGSQYQCENGVSGPSQDLLPPPNPYPLPQEHSQIYHCTKRKE |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | T-box 5 |
| 别名 | TBX5, T-box transcription factor TBX5, T-box protein 5, T box 5, HOS |
| 计算分子量 | 518 aa, 57 kDa |
| 观测分子量 | 58 kDa |
| GenBank蛋白编号 | BC027942 |
| 基因名称 | TBX5 |
| Gene ID (NCBI) | 6910 |
| RRID | AB_10638435 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q99593 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
T-box genes encode a family of transcription factors that have been identified in all metazoans and which play diverse roles during embryonic development. TBX5, a member of T-box family, has an essential role in the initiation of limb outgrowth and heart development. Genetic variation near TBX5 that associates with PR and/or QRS interval variation, which suggests that TBX5 plays a role in CCS function in the general population. Because 77 % amino acid sequence of TBX5 is identical to TBX4, this antibody recognizes both TBX4 and TBX5.
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文献和实验| Species | Application | Title |
|---|---|---|
| human | IF | Eur Respir J Single-cell RNA sequencing reveals that BMPR2 mutation regulates right ventricular function via ID genes.Authors - Mingxia Du |
| human | WB | Mol Biol Evol Functional and Adaptive Significance of Promoter Mutations that Affect Divergent Myocardial Expressions of TRIM72 in Primates.Authors - Yuanqing Feng |
| mouse | WB | Environ Sci Technol Perfluorinated Iodine Alkanes Promoted Neural Differentiation of mESCs by Targeting miRNA-34a-5p in Notch-Hes Signaling.Authors - Zhihua Ren |
Stem Cell Reports Soft Matrix Promotes Cardiac Reprogramming via Inhibition of YAP/TAZ and Suppression of Fibroblast Signatures.Authors - Shota Kurotsu | ||
| human | IF | iScience A dual SHOX2:GFP; MYH6:mCherry knockin hESC reporter line for derivation of human SAN-like cells.Authors - Zaniar Ghazizadeh |
| mouse | WB | Sci Rep Stoichiometric optimization of Gata4, Hand2, Mef2c, and Tbx5 expression for contractile cardiomyocyte reprogramming.Authors - Zhentao Zhang |
因此,综合来说,EarthOx的GST标签抗体 (货号#E022040)是一款同时具有高特异性、高灵敏度、多应用类型以及性价比的GST标签抗体。同时,我们也提供其他品牌的GST标签抗体,如Proteintech的GST多克隆抗体 ――GST PolyClonal Antibody(货号#10000-0-AP)以及Abcam、Santa等多种GST标签抗体产品。更多GST标签抗体以及其他如His、HA、Myc、GFP、Flag等其他标签抗体,请电话垂询。美国EarthOx品牌是一家提供高性价比免疫学和细胞
Microarray Approaches for Analysis of Cell Cycle Regulatory Genes
Cell cycle phase Mammalian Yeast G0/G1 AP-1 family (FOS, JUN)
同时,Apaf―1可以激活原形的caspase―9,形成细胞色素C/Apaf―1/caspase―9复合体,即凋亡复合体(apoptosome)。该复合体水解并激活下游的caspase通路如caspase―3等。 凋亡复合体的形成是凋亡信号启动的关键步骤。目前发现激活的癌基因可以辅助凋亡复合体的形成,比如腺病毒EIA可以上调Apaf―1和caspase―9,E2F1可以上调Apaf―1的表达。Apaf―1的释放可促进凋亡复合体的形成,对凋亡的启动起促进作用。目前的研究认为,细胞
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