- ￥1350 - 3000
- Proteintech
- 13111-1-AP
- 2025年10月13日
- IHC, ELISA
- Rabbit
- human
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- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
Recombinant human TNFB protein
- 亚型：
IgG
- 保存条件：
Store at -20°C. Stable for one year after shipment.
- 克隆性：
Polyclonal
- 标记物：
Unconjugated
- 适应物种：
human
- 保质期：
详见产品包装
- 库存：
充足
- 宿主：
Rabbit
- 应用范围：
IHC, ELISA
- 靶点：
TNF beta
- 抗体英文名：
TNF beta Polyclonal antibody
- 抗体名：
TNF beta Polyclonal antibody
- 规格：
50ul/100ul/150ul
| 规格： | 50ul | 产品价格： | ￥1350.0 |
|---|---|---|---|
| 规格： | 100ul | 产品价格： | ￥2200.0 |
| 规格： | 150ul | 产品价格： | ￥3000.0 |
经过测试的应用
| Positive IHC detected in | human malignant melanoma tissue, human testis tissue, human tonsillitis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
13111-1-AP targets TNF beta in WB, IHC, IF, ELISA applications and shows reactivity with human samples.
| 经测试应用 | IHC, ELISA |
| 文献引用应用 | WB, IF |
| 经测试反应性 | human |
| 文献引用反应性 | human, mouse |
| 免疫原 |
CatNo: Ag3775 Product name: Recombinant human TNFB protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 35-205 aa of BC034729 Sequence: LPGVGLTPSAAQTARQHPKMHLAHSTLKPAAHLIGDPSKQNSLLWRANTDRAFLQDGFSLSNNSLLVPTSGIYFVYSQVVFSGKAYSPKATSSPLYLAHEVQLFSSQYPFHVPLLSSQKMVYPGLQEPWLHSMYHGAAFQLTQGDQLSTHTDGIPHLVLSPSTVFFGAFAL |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | lymphotoxin alpha (TNF superfamily, member 1) |
| 别名 | LTA, LT alpha, TNFB, TNF β, Lymphotoxin-alpha |
| 计算分子量 | 205 aa, 22 kDa |
| GenBank蛋白编号 | BC034729 |
| 基因名称 | TNF beta |
| Gene ID (NCBI) | 4049 |
| RRID | AB_2877916 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | P01374 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
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文献和实验| Species | Application | Title |
|---|---|---|
| human | IF | Adv Sci (Weinh) Plac1+ Tumor Cell-Treg Interplay Supports Tumorigenesis and Progression of Head and Neck CancerAuthors - Xiaoyan Meng |
| human | IF | Diagnostics (Basel) Loss of Lymphotoxin Alpha-Expressing Memory B Cells Correlates with Metastasis of Human Primary Melanoma.Authors - Franziska Werner |
| mouse | WB | Phytomedicine Glycosides from Buyang Huanwu Decoction inhibit atherosclerotic inflammation via JAK/STAT signaling pathwayAuthors - Xinying Fu |
| human | IF | Br J Cancer Machine learning for the identification of neoantigen-reactive CD8 + T cells in gastrointestinal cancer using single-cell sequencingAuthors - Hongwei Sun |
Microbiol Res Androgen deficiency-induced loss of Lactobacillus salivarius extracellular vesicles is associated with the pathogenesis of osteoporosisAuthors - Wenjun Zhang |
Use of RNA Interference to Investigate Cytokine Signal Transduction in Pancreatic Beta Cells
to the cytokines interleukin(IL)-1β + interferon(IFN)-γ or to tumor necrosis factor(TNF)-α + IFN-γ induces beta cell dysfunction and ultimately apoptosis. The transcription factors NF-κB and STAT1 are key regulators of cytokine-induced beta cell death
2. 抗原与填料的连接 这一步是整个纯化过程中最关键的一步，如果在这一步上有什么失误，建议你从step one重新开始吧。 首先要注意一点：常见的连接方法都基于抗原上的游离氨基或者巯基与活化后的beads的连接，因此体系中不应该有其它含有氨基或巯基的化合物，包括尿素、硫脲、盐酸胍、Tris、铵根离子、Beta-巯基乙醇等，如果有这些物质在体系中，应该首先设法除去。 溴化氰活化的琼脂糖与氨基化合物反应的机理如下： 环氧氯丙烷活化的琼脂糖与氨基化合物反应
tumor necrosis factor–{alpha} (TNF-{alpha}), interferon-beta (IFN-beta), and IFN-{gamma} (1). The authors observed that of ~200 miRNAs examined, only miR-155 increased in amount in response to both TLR ligands and IFN-beta. miR-155 accumulated
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