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- 详细信息
- 文献和实验
- 技术资料
- 免疫原:
Recombinant human EPS8 protein
- 亚型:
IgG
- 保存条件:
Store at -20°C. Stable for one year after shipment.
- 克隆性:
Polyclonal
- 标记物:
Unconjugated
- 适应物种:
human
- 保质期:
详见产品包装
- 库存:
充足
- 宿主:
Rabbit
- 应用范围:
WB, IHC, IF/ICC, IP, ELISA
- 靶点:
EPS8
- 抗体英文名:
EPS8 Polyclonal antibody
- 抗体名:
EPS8 Polyclonal antibody
- 规格:
50ul/100ul/150ul
| 规格: | 50ul | 产品价格: | ¥1350.0 |
|---|---|---|---|
| 规格: | 100ul | 产品价格: | ¥2200.0 |
| 规格: | 150ul | 产品价格: | ¥3000.0 |
经过测试的应用
| Positive WB detected in | HEK-293T cells, HeLa cells |
| Positive IP detected in | HeLa cells |
| Positive IHC detected in | human breast cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | A375 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:10-1:100 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
12455-1-AP targets EPS8 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, IHC, IF/ICC, IP, ELISA |
| 文献引用应用 | WB, IHC |
| 经测试反应性 | human |
| 文献引用反应性 | human, mouse |
| 免疫原 |
CatNo: Ag3121 Product name: Recombinant human EPS8 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 13-267 aa of BC030010 Sequence: GMYPSQMNGYGSSPTFSQTDREHGSKTSAKALYEQRKNYARDSVSSVSDISQYRVEHLTTFVLDRKDAMITVDDGIRKLKLLDAKGKVWTQDMILQVDDRAVSLIDLESKNELENFPLNTIQHCQAVMHSCSYDSVLALVCKEPTQNKPDLHLFQCDEVKANLISEDIESAISDSKGGKQKRRPDALRMISNADPSIPPPPRAPAPAPPGTVTQVDVRSRVAAWSAWAADQGDFEKPRQYHEQEETPEMMAARID |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | epidermal growth factor receptor pathway substrate 8 |
| 别名 | Epidermal growth factor receptor kinase substrate 8 |
| 计算分子量 | 822 aa, 92 kDa |
| 观测分子量 | 97 kDa |
| GenBank蛋白编号 | BC030010 |
| 基因名称 | EPS8 |
| Gene ID (NCBI) | 2059 |
| RRID | AB_2098836 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q12929 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Epidermal growth factor receptor Pathway Substrate 8 (EPS8) is a crucial regulator of the actin cytoskeleton dynamics accompanying cell motility and invasion. This protein contains one PH domain and one SH3 domain leading to its binding activity with multiple cellular targets. EPS8 can function as a unique actin capping protein specifically required for dendritic cell migration and plays roles in the regulation of axonal filopodia in neuronal development and synapse formation. The EPS8 gene may contribute to the development of a subset of colorectal cancers and could have applications in diagnosis and treatment.
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文献和实验| Species | Application | Title |
|---|---|---|
| human | WB,IHC | Exp Cell Res EPS8 supports pancreatic cancer growth by inhibiting BMI1 mediated proteasomal degradation of ALDH7A1.Authors - Mingzhu Tan
|
| human | WB | Acta Biochim Biophys Sin (Shanghai) miR-345 inhibits migration and stem-like cell phenotype in gastric cancer via inactivation of Rac1 by targeting EPS8.Authors - Jieyun Zhang
|
| mouse | WB | Acta Otolaryngol The 133-kDa N-terminal region of myosin XVa is critical for normal structure and function of auditory and hair cellsAuthors - Yong Li |
重大突破!马光辉/魏炜/李玉华团队 Nature 子刊报道新型精准治疗性白血病疫苗
Biomedical Engineering研究内容作者的前期临床研究结果表明,EPS8 和 PD-1/PD-L1 在白血病患者中高表达,可以分别用作新型白血病抗原和白血病疫苗的检查点靶标。 图片来源:Nature Biomedical Engineering由于会被快速清除,用 EPS8 抗原肽和 PD-1 抗体(免疫检查点抑制剂)混合直接接种的效果并不好。因此,作者利用自愈合大孔微球构建了疫苗递送新剂型,其新颖的「后包埋」策略实现了抗原肽和抗体的高效装载,并充分保护了二者的活性。这种自愈合大孔微球
因此,综合来说,EarthOx的GST标签抗体 (货号#E022040)是一款同时具有高特异性、高灵敏度、多应用类型以及性价比的GST标签抗体。同时,我们也提供其他品牌的GST标签抗体,如Proteintech的GST多克隆抗体 ――GST PolyClonal Antibody(货号#10000-0-AP)以及Abcam、Santa等多种GST标签抗体产品。更多GST标签抗体以及其他如His、HA、Myc、GFP、Flag等其他标签抗体,请电话垂询。美国EarthOx品牌是一家提供高性价比免疫学和细胞
实验,请根据说明书列出已验证的种属来选择适合你实验的抗体。 (4)样本蛋白的结构性质。了解样本蛋白的结构性质有助于选择最合适的抗体,待测样本蛋白的结构域和样本在提取和处理过程中是否会变性,蛋白空间构象的改变,会影响抗体的免疫亲和反应。 (5)单多克隆抗体的选择。市面上的抗体还是以多克隆抗体为主,一般单克隆抗体特异性强,但亲和力相对小,检测抗原灵敏度相对就低;而多克隆抗体特异性稍弱,但抗体的亲和力强,灵敏度高,但易出现非特异性染色(可以通过封闭等避免
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