- ￥1350 - 3000
- Proteintech
- 12227-1-AP
- 2025年10月13日
- WB, IP, IHC, ELISA
- Rabbit
- human, mouse, rat
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- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
Recombinant human EIF4E2 protein
- 亚型：
IgG
- 保存条件：
Store at -20°C. Stable for one year after shipment.
- 克隆性：
Polyclonal
- 标记物：
Unconjugated
- 适应物种：
human, mouse, rat
- 保质期：
详见产品包装
- 库存：
充足
- 宿主：
Rabbit
- 应用范围：
WB, IP, IHC, ELISA
- 靶点：
EIF4E2
- 抗体英文名：
EIF4E2 Polyclonal antibody
- 抗体名：
EIF4E2 Polyclonal antibody
- 规格：
50ul/100ul/150ul
| 规格： | 50ul | 产品价格： | ￥1350.0 |
|---|---|---|---|
| 规格： | 100ul | 产品价格： | ￥2200.0 |
| 规格： | 150ul | 产品价格： | ￥3000.0 |
经过测试的应用
| Positive WB detected in | HeLa cells, MCF-7 cells, mouse kidney tissue |
| Positive IP detected in | MCF-7 cells |
| Positive IHC detected in | human breast cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
12227-1-AP targets EIF4E2 in WB, IP, RIP, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IP, IHC, ELISA |
| 文献引用应用 | WB, RIP, IHC |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human |
| 免疫原 |
CatNo: Ag2864 Product name: Recombinant human EIF4E2 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-245 aa of BC021690 Sequence: MNNKFDALKDDDSGDHDQNEENSTQKDGEKEKTERDKNQSSSKRKAVVPGPAEHPLQYNYTFWYSRRTPGRPTSSQSYEQNIKQIGTFASVEQFWRFYSHMVRPGDLTGHSDFHLFKEGIKPMWEDDANKNGGKWIIRLRKGLASRCWENLILAMLGEQFMVGEEICGAVVSVRFQEDIISIWNKTASDQATTARIRDTLRRVLNLPPNTIMEYKTHTDSIKMPGRLGPQRLLFQNLWKPRLNVP |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | eukaryotic translation initiation factor 4E family member 2 |
| 别名 | 4E LP, 4EHP, eIF 4E type 2, eIF4E like protein 4E LP, eIF4E type 2, EIF4E2, EIF4EL3, IF4e, mRNA cap binding protein 4EHP |
| 计算分子量 | 245 aa, 28 kDa |
| 观测分子量 | 28 kDa |
| GenBank蛋白编号 | BC021690 |
| 基因名称 | EIF4E2 |
| Gene ID (NCBI) | 9470 |
| RRID | AB_10642945 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | O60573 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
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文献和实验| Species | Application | Title |
|---|---|---|
| human | WB | Nat Commun Stalled translation by mitochondrial stress upregulates a CNOT4-ZNF598 ribosomal quality control pathway important for tissue homeostasisAuthors - Ji Geng |
| human | WB,RIP | Cell Death Dis α1,3-fucosylation of MEST promotes invasion potential of cytotrophoblast cells by activating translation initiationAuthors - Hao Wang |
| human | WB | iScience The SARS-CoV-2 protein NSP2 impairs the silencing capacity of the human 4EHP-GIGYF2 complex.Authors - Limei Zou |
| human | WB,IHC | Eur J Med Res N7-methylguanosin regulators-mediated methylation modification patterns and characterization of the immune microenvironment in lower-grade gliomaAuthors - Aierpati Maimaiti |
| human | WB | Nat Struct Mol Biol Isoform-resolved mRNA profiling of ribosome load defines interplay of HIF and mTOR dysregulation in kidney cancerAuthors - Yoichiro Sugimoto |
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