- ￥1350 - 3000
- Proteintech
- 12192-1-AP
- 2025年10月13日
- WB, IHC, FC (Intra), IP, ELISA
- Rabbit
- human
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- 详细信息
- 询价记录
- 文献和实验
- 技术资料
- 免疫原：
Recombinant human SERPINA3,AACT protein
- 亚型：
IgG
- 保存条件：
Store at -20°C. Stable for one year after shipment.
- 克隆性：
Polyclonal
- 标记物：
Unconjugated
- 适应物种：
human
- 保质期：
详见产品包装
- 库存：
充足
- 宿主：
Rabbit
- 应用范围：
WB, IHC, FC (Intra), IP, ELISA
- 靶点：
Alpha 1 antichymotrypsin
- 抗体英文名：
Alpha 1 antichymotrypsin Polyclonal antibody
- 抗体名：
Alpha 1 antichymotrypsin Polyclonal antibody
- 规格：
50ul/100ul/150ul
| 规格： | 50ul | 产品价格： | ￥1350.0 |
|---|---|---|---|
| 规格： | 100ul | 产品价格： | ￥2200.0 |
| 规格： | 150ul | 产品价格： | ￥3000.0 |
经过测试的应用
| Positive WB detected in | human blood tissue, COLO 320 cells, human colon tissue |
| Positive IP detected in | COLO 320 cells |
| Positive IHC detected in | human tonsillitis tissue, human liver tissue, human spleen tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive FC (Intra) detected in | HepG2 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
12192-1-AP targets Alpha 1 antichymotrypsin in WB, IHC, IF, FC (Intra), IP, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, IHC, FC (Intra), IP, ELISA |
| 文献引用应用 | WB, IHC, IF |
| 经测试反应性 | human |
| 文献引用反应性 | human, mouse, rat |
| 免疫原 |
CatNo: Ag2830 Product name: Recombinant human SERPINA3,AACT protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 70-423 aa of BC010530 Sequence: DKNVIFSPLSISTALAFLSLGAHNTTLTEILKGLKFNLTETSEAEIHQSFQHLLRTLNQSSDELQLSMGNAMFVKEQLSLLDRFTEDAKRLYGSEAFATDFQDSAAAKKLINDYVKNGTRGKITDLIKDLDSQTMMVLVNYIFFKAKWEMPFDPQDTHQSRFYLSKKKWVMVPMMSLHHLTIPYFRDEELSCTVVELKYTGNASALFILPDQDKMEEVEAMLLPETLKRWRDSLEFREIGELYLPKFSISRDYNLNDILLQLGIEEAFTSKADLSGITGARNLAVSQVVHKAVLDVFEEGTEASAATAVKITLLSALVETRTIVRFNRPFLMIIVPTDTQNIFFMSKVTNPKQA |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | serpin peptidase inhibitor, clade A (alpha-1 antiproteinase, antitrypsin), member 3 |
| 别名 | Alpha Antichymotrypsin, SERPINA3, Alpha-1-antichymotrypsin, ACT, AACT |
| 计算分子量 | 448 aa, 48 kDa |
| 观测分子量 | 44-70 kDa |
| GenBank蛋白编号 | BC010530 |
| 基因名称 | Alpha 1 Antichymotrypsin |
| Gene ID (NCBI) | 12 |
| RRID | AB_10344682 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | P01011 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Human SerpinA3, also known as Alpha 1-antichymotrypsin (AACT), is a member of the serine protease inhibitor class, and is a plasma alpha globulin glycoprotein which increases in the blood during the inflammatory process. SerpinA3, is also an inhibitor of neutrophil cathepsin G, mast cell chymases and pancreatic chymotrypsin. SerpinA3 is produced primarily in the liver, and is identified as an acute-phase inflammatory protein. SerpinA3 deficiency has been associated with liver disease. SerpinA3 has also been implicated in the pathology of a number of devastating human diseases including chronic obstructive pulmonary disease (COPD), Parkinson's disease (PD), Alzheimer's disease (AD), Stroke, Cystic Fibrosis, Cerebral Haemorrhage and Multiple System Atrophy.
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- 作者
- 内容
- 询问日期
文献和实验| Species | Application | Title |
|---|---|---|
| mouse | WB | Nat Metab Maternal exercise prevents metabolic disorders in offspring mice through SERPINA3CAuthors - Yang Li |
Acta Neuropathol Integrative proteomics highlight presynaptic alterations and c-Jun misactivation as convergent pathomechanisms in ALSAuthors - Amr Aly | ||
| human | WB | Front Immunol Screening and identification of tissue-infiltrating immune cells and genes for patients with emphysema phenotype of COPDAuthors - Di Wang |
| human | WB,IHC | Br J Cancer Identification of GlcNAcylated alpha-1-antichymotrypsin as an early biomarker in human non-small-cell lung cancer by quantitative proteomic analysis with two lectins.Authors - Yanxia Jin |
| mouse | IHC | Front Med (Lausanne) An Integrated Proteomics and Metabolomics Strategy for the Mechanism of Calcium Oxalate Crystal-Induced Kidney Injury.Authors - Songyan Gao |
Proteomics Clin Appl Serum protein profiling in diffuse large B-cell lymphoma.Authors - Jacques Riby |
）、Anti-IgA （alpha）】 在一些特殊的实验里，只需要检测样本里的IgG或者IgM，常规的Anti-IgG （H+L）由于与IgG的重链和轻链都有结合反应，而IgG、IgM、IgA都有保守的轻链结构域，因此不能特异性的检测某种类型的IgG或者IgM。这时候就需要针对特殊类型Ig分子的二抗。***捷备有全面的二抗现货产品并能提供最完整的二抗产品线。主要二抗品牌为全球最大的二抗产品Jackson二抗以及前沿抗体生产商EarthOx。按照标记分，有HRP、AP、生物素等标记二抗，FITC
隆抗体，二抗则选抗小鼠的二抗（山羊抗小鼠或者兔抗小鼠等均可）；如果一抗是从兔血清里制备的兔源多克隆抗体，则相应的二抗需要选择抗兔的二抗。即根据一抗的物种来源选择相应的抗该物种的二抗。艾美捷能为您提供最全的抗不同种属的原装进口二抗，包括抗小鼠、大鼠、兔、山羊、绵羊、人、豚鼠、猪、马、牛、鸡、鸭等二抗。 【一抗的类别亚型】 二抗需与一抗的类别或亚类相匹配。这通常是针对单克隆抗体而言。多克隆抗体主要是IgG类免疫球蛋白，因此相应的二抗就是抗IgG抗体。其中单克隆抗体的类别及亚类通常会在产品说明书中都会
可）；如果一抗是从兔血清里制备的兔源多克隆抗体，则相应的二抗需要选择抗兔的二抗。即根据一抗的物种来源选择相应的抗该物种的二抗。艾美捷能为您提供最全的抗不同种属的原装进口二抗，包括抗小鼠、大鼠、兔、山羊、绵羊、人、豚鼠、猪、马、牛、鸡、鸭等二抗。【一抗的类别亚型】二抗需与一抗的类别或亚类相匹配。这通常是针对单克隆抗体而言。多克隆抗体主要是IgG类免疫球蛋白，因此相应的二抗就是抗IgG抗体。其中单克隆抗体的类别及亚类通常会在产品说明书中都会有描述，如果你的一抗是小鼠IgM，那么相应的二抗就应当是抗小鼠IgM
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